Induction of Proline Iminopeptidase by Nitric Oxide May Result in the Proline Accumulation in the Pea Roots.

The nitric oxide (NO) donor sodium nitroprusside upregulated the proline iminopeptidase content in the pea seedling roots. It is assumed that NO activates deprolinization of the proline-rich proteins, as evidenced by an increase in the content of free proline, which is known to protect plants from the abiotic and biotic stressors.

Salicylate-Induced Chitinases in Pea Roots.

Three proteins induced by salicylic acid were revealed in pea roots. These proteins were identified as chitinase isozymes belonging to the glycoside hydrolases family 18. The PsCam050724 transcript encoding at least one of these isoforms was found, allowing us to determine its primary structure, which lacks the signal peptide.

Azelaic Acid-Induced Enzymes of Phenolic Defense in Pea Roots.

The treatment of pea roots with azelaic acid for 72 h led to a change in the content of 28 proteins: the content of 20 proteins decreased, and the content of 8 proteins (including the phenylpropanoid metabolism enzymes, which are involved in the synthesis of phytoalexins) increased.