From Division to Death: Metabolomic Analysis of BY-2 Cells Reveals the Complexity of Life in Batch Culture.

Tobacco BY-2 cell culture is one of the most widely used models in plant biology. The main advantage of BY-2 suspension cultures is the synchronization of cell development and the appearance of polar elongation. In batch culture, BY-2 cells passed through the lag, proliferation, elongation, and stationary phases.

Uptake and Metabolic Conversion of Exogenous Phosphatidylcholines Depending on Their Acyl Chain Structure in .

Fungi and plants are not only capable of synthesizing the entire spectrum of lipids de novo but also possess a well-developed system that allows them to assimilate exogenous lipids. However, the role of structure in the ability of lipids to be absorbed and metabolized has not yet been characterized in detail. In the present work, targeted lipidomics of phosphatidylcholines (PCs) and phosphatidylethanolamines (PEs), in parallel with morphological phenotyping, allowed for the identification of differences in the effects of PC molecular species introduced into the growth medium, in particular, typical bacterial saturated (14:0/14:0, 16:0/16:0), monounsaturated (16:0/18:1), and typical for fungi and plants polyunsaturated (16:0/18:2, 18:2/18:2) species, on .

Antioxidant and cytotoxic activities of quinones from .

Chemical investigation of the extracts obtained from the red thallus tips from resulted in the isolation of five known quinoid pigments identified by FT-IR, UV, NMR, MS methods and by comparison with literature data (skyrin (), 3-ethyl-2,7-dihydroxynaphthazarin (), graciliformin (), cuculoquinone () and islandoquinone ()). An antioxidant capacity of compounds - were evaluated and compared with quercetin using a lipid peroxidation inhibitory assay and superoxide radical (SOR), nitric oxide radical (NOR), 1,1-diphenyl-2-picrylhydrazine (DPPH), 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonate) (ABTS) scavenging assays. Compounds and were far more active: they demonstrated the antioxidant capacity in various test assays with the IC values 5-409 µM comparable to the flavonoid quercetin.

Genotoxicity of (+)- and (-)-usnic acid in mice.

Usnic acid, which is the most widespread and well-studied secondary lichen compound, has antibacterial and cytotoxic effects. Usnic acid is present in lichens as the (+)- and (-)-enantiomers, which have different biological activities. We used a DNA-comet assay to determine the genotoxic effect of (+)- and (-)-usnic acid in the liver and kidney cells of mice.

GENOTOXIC EFFECT OF USNIC ACID ENANTIOMERS IN VITRO IN HUMAN PERIPHERAL BLOOD LYMPHOCYTES.

Cytotoxic and genotoxic effect of (+) and (–) enantiomers of usnic acid in human peripheral blood lymphocytes studed. We have shown that the studied usnic acid enantiomers in concentrations of 0.04—0.