Risk factors for post-COVID-19 condition in previously hospitalised children using the ISARIC Global follow-up protocol: a prospective cohort study.

Background: The long-term sequelae of coronavirus disease 2019 (COVID-19) in children remain poorly characterised. This study aimed to assess long-term outcomes in children previously hospitalised with COVID-19 and associated risk factors.

Methods: This is a prospective cohort study of children (≤18 years old) admitted to hospital with confirmed COVID-19.

A metabolic engineering strategy for producing free fatty acids by the Yarrowia lipolytica yeast based on impairment of glycerol metabolism.

In recent years, bio-based production of free fatty acids from renewable resources has attracted attention for their potential as precursors for the production of biofuels and biochemicals. In this study, the oleaginous yeast Yarrowia lipolytica was engineered to produce free fatty acids by eliminating glycerol metabolism. Free fatty acid production was monitored under lipogenic conditions with glycerol as a limiting factor.

Methods coagulation/flocculation and flocculation with ballast agent for effective harvesting of microalgae.

The effects of coagulant (FeCl3·6H2O), various flocculants based on polyacrylamide (PAA), polyethylenoxide (PEO) and flocculated biomass as ballast agent, dosage and sedimental time on flocculation efficiency of harvesting Chlorella vulgaris GKV1 cultivated in a laboratory were investigated. The results of this work indicated that the flocculation efficiency achieved about 90% after 5 min of sedimentation when adding of coagulant and flocculant mixture (FeCl3 50 mg/l+PEO based Sibfloc-718 7.5 mg/l) or flocculant with ballast agent (Sibfloc-718 7.

[Number, viability, and diversity of the filterable forms of prokaryotes in sphagnous high-moor peat].

The number, potential viability, and taxonomic diversity (at the level of phylum) of the filterable forms of prokaryotes (FFP) are estimated in the main genetic horizons of high-moor peat. It was shown that the number of FFP reached 500 million cells in 1 g, i.e.

[Definition of the physiological condition of bacteria in soil by means of luminescent dye L7012].

By means of dye L7012, the number and physiological condition (damage rate of membranes) of bacterial cells is defined. The results testify to considerable physiological heterogeneity of bacteria cells in soils. In fresh samples of soil, the percentage of intact cells reached 60-70%.