Publications by authors named "I A Gusev"
Article Synopsis
- The study analyzed the genetic makeup of Orenburg goats using SNP data from modern and historical samples, revealing that the genetic characteristics from older populations have persisted in current goats.
- Findings indicated low inbreeding rates and maintained genetic diversity, providing a basis for selective breeding and potential conservation strategies through gamete preservation.
Intrauterine hypoxia (gestation days 15-19, pO 65 mm Hg, duration 4 h) led to an increase in the expression of p53, beclin-1, endothelial NO synthase (eNOS), and caspase-3 proteins in cardiomyocytes and reduced the number of mast cells in the heart of 60-day-old albino rats. Administration of a non-opiate analogue of leu-enkephalin (NALE peptide: Phe-D-Ala-Gly-Phe-Leu-Arg, 100 μg/kg) on days 2-6 of the neonatal period decreased the severity of delayed posthypoxic myocardial reaction. The content of eNOS cardiomyocytes and the total number of mast cells of these animals did not differ from the control parameters; the content of p53 cardiomyocytes was significantly lower than in animals exposed to intrauterine hypoxia.
View Article and Find Full Text PDFAcute exposure of isolated ventricular cardiomyocytes to non-opiate analogue of leu-enkephalin (NALE peptide: Phe-D-Ala-Gly-Phe-Leu-Arg) in a concentration of 100 μg/liter and 6-h incubation in NALE solution did not significantly change ATP-dependent K current, L-type Ca current, p53 protein expression, and number of nucleoli in the cardiomyocyte nuclei. Incubation of cardiomyocytes with NALE (100 μg/liter) in combination with NOP receptor blocker J-113397 (1 mg/liter) was followed by an increase in Ca L-type current and the number of p53 cells. The exposure of cardiomyocytes to NALE in a concentration 1000 μg/liter induced similar changes in the studied parameters (increase in Ca L-type current and number of p53 cardiomyocytes); an increase in the mean number of nucleoli was also observed.
View Article and Find Full Text PDFArticle Synopsis
- The text discusses the impact of evolutionary and environmental factors on the genomes of various poultry breeds, particularly in relation to cold tolerance and performance traits.
- Using a specialized SNP chip, researchers genotyped four different chicken breeds and identified genomic regions indicative of selection for cold adaptation through multiple analysis methods.
- The study highlights several key candidate genes linked to cold tolerance and suggests that both genetic and epigenetic factors may play roles in how these breeds adapt to harsh climates, pointing to the need for more research on these mechanisms.
Hypobaric hypoxia (pO 65 mm Hg, duration 4 h) induced a significant increase in the number of cardiomyocytes expressing р53, beclin-1, endothelial NO synthase and accumulation and degranulation of mast cells in the epicardium in hearts of prepubertal female rats (age 45-47 days); the number of cardiomyocytes with nucleoli decreased, while the number of single-nucleolar cardiomyocytes increased after this exposure. Five-fold administration of non-opiate analogue of leu-enkephalin (NALE peptide: Phe-D-Ala-Gly-Phe-Leu-Arg; 100 μg/kg) during the neonatal period reduced the severity of the post-hypoxic changes in the heart. Neonatal administration of NALE (100 μg/kg) against the background of NO synthase blockade with L-NAME (50 mg/kg) did not abolish the cardioprotective effects of the peptide.
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