Regulation of Peptide Liquid-Liquid Phase Separation by Aromatic Amino Acid Composition.

Membraneless organelles are cellular biomolecular condensates that are formed by liquid-liquid phase separation (LLPS) of proteins and nucleic acids. LLPS is driven by multiple weak attractive forces, including intermolecular interactions mediated by aromatic amino acids. Considering the contribution of π-electron bearing side chains to protein-RNA LLPS, systematically study sought to how the composition of aromatic amino acids affects the formation of heterotypic condensates and their physical properties.

Genetic Determinants of Macrolide and Fluoroquinolone Resistance in and Their Prevalence in Moscow, Russia.

Macrolide (MLR) and fluoroquinolone (FQR) resistance in (MG) has recently become a major problem worldwide. The available data on the prevalence of MLR and FQR in MG in Russia are limited. In this study, we aimed to evaluate the prevalence and pattern of mutations in 213 MG-positive urogenital swabs from patients in Moscow between March 2021 and March 2022.

Development of a Real-Time PCR Assay for Detection of Macrolide Resistance Mutations in and Its Application for Epidemiological Surveillance in Russia.

A real-time PCR assay based on probe fluorescence quenching by a primer was developed and validated for detection of macrolide resistance (MR)-associated mutations in the 23S rRNA gene of The assay involves identification of any nucleotide substitutions at positions 2058, 2059, and 2611 of 23S rRNA ( numbering) by the probe-based melting curve analysis immediately after amplification and was capable of detecting target mutations in clinical specimens and spiked samples with 92% sensitivity and 100% specificity. We applied this new assay to assess the prevalence of MR-associated mutations in 949 nonduplicate urogenital samples positive for by routine diagnostic PCR, which were collected from symptomatic patients in five cities in the European part of Russia during the period 2009-2019. Forty-three (4.

Course of Lower Respiratory Tract Infection in Young People Treated at the Military Hospital of Smolensk Garrison with Detected Carrying a Macrolide-Resistant Mutation in 23S rRNA Gene.

We evaluated the effect of macrolide-resistant mutations in the Mycoplasma pneumoniae 23S rRNA gene on the severity of lower respiratory tract infections in immunocompetent young adults treated at the Smolensk Military Hospital between 25 October 2017, and 17 November 2021. All analyzed cases represented a non-severe infection of the lower respiratory tract: 44 case histories with community-acquired pneumonia and 20 cases with acute bronchitis. The presence of mutations in the gene 23S rRNA of M.

Is green space associated with reduced crime? A national-scale study from the Global South.

Assumptions about the link between green space and crime mitigation are informed by literature that overwhelmingly originates in the Global North. Little is known about the association between green spaces and crime in the Global South. We utilized 10 years of precinct-level crime statistics (n = 1152) over South Africa, a global crime hotspot, to test the hypothesis that green space is associated with reduced crime rates.