[Laboratory errors for identification of the escherichia coli strains of the serological groups O6 and O25 as acute intestinal infections.].

Were studied the genes encoding the virulence factors of 221 strains: E. coli O:H (194) and E. coli O:H (27), isolated in 2014-2018 from stool samples of children and adults examined according to epidemic indications.

[Stimulating effect of polyion on the production of cytolytic T lymphocytes in a cell culture in vitro].

Introduction of polyion "vegetan" at the concentrations varying from 20 to 100 micrograms/ml into mixed lymphocyte culture (MLC) with insufficient antigenic stimulus, low immunogenic MLC, for the whole of the incubation period, leads to 30-70% increase in T-lymphocyte cytolytic activity. The effect of vegetan on the cytolytic activity of T-lymphocytes from normal MLC is less marked. In the in vivo model of primary synthesis of antibodies against heterologous erythrocyte antigens, vegetant causes 20-50-fold enhancement of a weak immune response compared to 1.

[Effect of myelopeptides on the cytolytic activity of T-lymphocytes].

To estimate myelopeptide effect on T-cell immunity a model system-mixed lymphocyte culture (MLC) with routine (1:1) and insufficient (20:1) ratio of reacting and stimulating cells was used. The latter culture was termed weakly immunogenic MLC. Myelopeptides administered into the weakly immunogenic MLC at the beginning of incubation ("0" period) at a concentration of 30 to 90 mkg/ml were shown to increase T-lymphocyte cytolytic activity by 35-50% without affecting cell proliferation.

[Interaction of diS-C3-(5) and ethylrhodamine with lymphocyte mitochondria].

The interaction of thymocyte mitochondria with two types of dyes - potential indicators commonly used in lymphocyte studies, has been investigated. Ethylrhodamine at concentrations up to 16 microM does not influence the systems of oxidation and energy coupling in lymphocyte mitochondria. Carbocyanines-diS-C3-(5) and diO-C3-(5) inhibit oxygen uptake by the lymphocytes in the presence of glucose and pyruvate at the same low concentrations as does rotenone (40% inhibition occurs at 10 nM).