Complete genome sequence of strain JD2-26, a potential pollutant-reducing bacterium isolated from a municipal solid waste landfill facility.

We report here the complete genome sequence of strain JD2-26 isolated from a municipal solid waste landfill facility. The genome consists of a 6.32-Mbp chromosome and a plasmid having a total of 6,100 genes, including 5,914 coding sequences.

sp. nov., isolated from seawater of the Yellow Sea.

A Gram-stain-negative and facultative aerobic bacterium, strain 16-MA, was isolated from seawater of Yellow Sea in South Korea. Cells were catalase- and oxidase-positive and non-motile rods. Growth occurred at 4-37 °C (optimum, 30 °C) and pH 6.

Complete Genome Sequence of a Sulfur-Oxidizing Bacterium, Alcaligenes faecalis Strain NLF5-7, Isolated from Livestock Wastewater.

The complete genome sequence of Alcaligenes faecalis strain NLF5-7, which was isolated from livestock wastewater, is reported. The genome of strain NLF5-7 contains genes for assimilatory sulfate reduction, dissimilatory sulfate reduction and oxidation, and an SOX system, based on its functional genetic characteristics.

Draft Genome Sequence of a Sulfur-Oxidizing Bacterium, Aquamicrobium lusatiense NLF2-7, Isolated from Livestock Wastewater.

Here, we report the draft genome sequence of Aquamicrobium lusatiense NLF2-7, a Gram-negative, aerobic, non-flagellum-forming, rod-shaped bacterium that was isolated from livestock wastewater in South Korea. The assembled genome sequence is 5,201,486 bp, with 4,972 protein-coding sequences in 12 contigs, and possess the genes for the sulfur oxidation pathway.

sp. nov., a benzophenone-3-degrading bacterium, isolated from stream sediment.

A Gram-stain-positive, facultative aerobic, oxidase-negative, catalase-positive, non-sporulating, and non-motile bacterium, which degraded benzophenone-3, was isolated from stream sediment collected in the Republic of Korea and designated as strain S2-17. Cells of this strain were rod-shaped during the early growth phase but became coccoid after the late exponential growth phase. Bacterial growth was observed at 15-37 °C (optimum, 25-30 °C) and pH 6.

Elucidating the biodegradation pathway and catabolic genes of benzophenone-3 in Rhodococcus sp. S2-17.

A new bacterium, Rhodococcus sp. S2-17, which could completely degrade an emerging organic pollutant, benzophenone-3 (BP-3), was isolated from contaminated sediment through an enrichment procedure, and its BP-3 catabolic pathway and genes were identified through metabolic intermediate and transcriptomic analyses and biochemical and genetic studies. Metabolic intermediate analysis suggested that strain S2-17 may degrade BP-3 using a catabolic pathway progressing via the intermediates BP-1, 2,4,5-trihydroxy-benzophenone, 3-hydroxy-4-benzoyl-2,4-hexadienedioic acid, 4-benzoyl-3-oxoadipic acid, 3-oxoadipic acid, and benzoic acid.

Complete Genome Sequence of Lacticaseibacillus paracasei Strain NSMJ15.

We report here the complete genome sequence of Lacticaseibacillus paracasei NSMJ15, isolated from makgeolli (a traditional Korean fermented liquor) and shown to have potentially probiotic characteristics. The genome consisted of a 2.79-Mbp chromosome contig and four plasmids having a total of 2,947 genes, including 2,690 coding sequences.

In-Vitro Characterization of Growth Inhibition against the Gut Pathogen of Potentially Probiotic Lactic Acid Bacteria Strains Isolated from Fermented Products.

Lactic acid bacteria (LAB) are probiotic candidates that may restore the balance of microbiota populations in intestinal microbial ecosystems by controlling pathogens and thereby promoting host health. The goal of this study was to isolate potential probiotic LAB strains and characterize their antimicrobial abilities against pathogens in intestinal microbiota. Among 54 LAB strains isolated from fermented products, five LAB strains (NSMJ15, NSMJ16, NSMJ23, NSMJ42, and NFFJ04) were selected as potential probiotic candidates based on in vitro assays of acid and bile salt tolerance, cell surface hydrophobicity, adhesion to the intestinal epithelium, and antagonistic activity.

sp. nov., isolated from soil.

A strict aerobic bacterium, strain JW14 was isolated from soil in the Republic of Korea. Cells were Gram-stain-positive, non-endospore-forming and motile rods showing catalase-positive and oxidase-negative activities. Growth of strain JW14 was observed at 20-37 °C (optimum, 30 °C), pH 6.

sp. nov., a lactic acid bacterium isolated from animal feed.

A Gram-positive, facultative anaerobic, catalase-negative, non-motile, non-spore-forming and rod-shaped lactic acid bacterium strain, denoted as NFFJ11 and isolated from total mixed fermentation feed in the Republic of Korea, was characterized through polyphasic approaches, including sequence analyses of the 16S rRNA gene and housekeeping genes ( and ), determination of average nucleotide identity and DNA-DNA hybridization, fatty acid methyl ester analysis, and phenotypic characterization. Phylogenetic analyses based on 16S rRNA, and gene sequences revealed that strain NFFJ11 belonged to the genus . The 16S rRNA gene sequence of strain NFFJ11 exhibited high similarity to S215 (99.

Complete Genome Sequence of Bisphenol A-Degrading Bacterium sp. Strain A3, Isolated from Contaminated Soil.

This study reports the complete genome sequence of bisphenol A-degrading bacterium sp. strain A3, which was isolated from a contaminated soil sample from the site of a factory fire in South Korea. The genome consists of a 6.

Complete Genome Sequence of Gordonia rubripertincta SD5, a Soil Bacterium Isolated from a Di-(2-Ethylhexyl) Phthalate-Degrading Enrichment Culture.

We report the complete genome sequence of SD5, isolated from a soil-derived di-(2-ethylhexyl) phthalate-degrading enrichment culture. The final genome assembly consists of a 5.10-Mbp chromosome and a plasmid (159 kbp).

Complete Genome Sequence of Lactobacillus harbinensis Strain NSMJ42, Isolated from Makgeolli, a Traditional Korean Alcoholic Beverage.

In the present work, we report the complete genome sequence of NSMJ42, isolated from makgeolli (a Korean traditional alcoholic beverage) in South Korea. The final genome assembly consists of a 3.29-Mbp chromosome with 3,082 protein-coding sequences and a G+C content of 53.

sp. nov., isolated from a culture of .

A Gram-stain-negative, yellow-pigmented, strictly aerobic bacterium, designated strain MaA-Y11, was isolated from a culture of in the Republic of Korea. Cells were non-motile rods showing catalase- and oxidase-positive reactions. Growth of strain MaA-Y11 was observed at 25-37 °C (optimum, 30 °C) and pH 6.

Complete Genome Sequence of Leuconostoc kimchii Strain NKJ218, Isolated from Homemade Kimchi.

strain NKJ218 was isolated from homemade kimchi in South Korea. The whole genome was sequenced using the PacBio RS II and Illumina NovoSeq 6000 platforms. Here, we report a genome sequence of strain NKJ218, which consists of a 1.

Sphingobacterium humi sp. nov., isolated from soil.

A Gram-stain-negative, facultatively aerobic bacterium, designated strain D1, was isolated from soil in South Korea. Cells of strain D1 were non-motile rods with oxidase- and catalase-positive activities. Growth was observed at 15-40 °C (optimum, 30-37 °C), at pH 5.

Cloacibacterium caeni sp. nov., isolated from activated sludge.

A Gram-stain-negative, facultatively anaerobic bacterium, designated B6T, was isolated from activated sludge of a wastewater treatment plant in South Korea. Cells were oxidase- and catalase-positive and non-motile rods producing yellow carotenoid-type pigments. Growth of B6T was observed at 20-40 °C (optimum, 37 °C) and pH 6.

Draft Genome Sequence of Zhouia amylolytica AD3, Isolated from Tidal Flat Sediment.

Zhouia amylolytica AD3 was isolated from tidal flat sediment at Taean, South Korea. We report here the draft genome sequence of Z. amylolytica AD3, which is the first report of a genome sequence of the genus Zhouia The genomic information will provide a better understanding of the physiology, adaptation, and evolution of Zhouia species.

Complete genome sequence of Sphingorhabdus sp. M41, a versatile hydrocarbon degrader, isolated from crude oil-contaminated costal sediment.

Sphingorhabdus sp. M41, capable of degrading aliphatic and aromatic hydrocarbons, was isolated from crude oil-contaminated costal sediment by an enrichment culture and its complete genome was sequenced. The genome of strain M41 has a chromosome with a size of 3,324,420bp, including 44 tRNAs, 6 rRNAs, and 3118 protein-coding genes.

Genome-wide transcriptional responses of Alteromonas naphthalenivorans SN2 to contaminated seawater and marine tidal flat sediment.

A genome-wide transcriptional analysis of Alteromonas naphthalenivorans SN2 was performed to investigate its ecophysiological behavior in contaminated tidal flats and seawater. The experimental design mimicked these habitats that either added naphthalene or pyruvate; tidal flat-naphthalene (TF-N), tidal flat-pyruvate (TF-P), seawater-naphthalene (SW-N), and seawater-pyruvate (SW-P). The transcriptional profiles clustered by habitat (TF-N/TF-P and SW-N/SW-P), rather than carbon source, suggesting that the former may exert a greater influence on genome-wide expression in strain SN2 than the latter.

Maribacter lutimaris sp. nov., isolated from marine sediment.

A Gram-staining-negative, moderately halophilic and strictly aerobic bacterium, designated strain KJ4T, was isolated from marine sediment at Gangjin in South Korea. Cells were catalase- and oxidase-positive long rods with gliding motility. Growth of strain KJ4T was observed at 4-37 °C (optimum, 15-25 °C), at pH 6.

sp. nov., isolated from estuary sediment.

A novel species of the genus , represented by strain S2-21, was isolated from an estuary of Asan in South Korea. Cells of strain S2-21 were Gram-stain-negative, aerobic, non-motile rods that were oxidase- and catalase-positive. Growth of strain S2-21 was observed at 15-40 °C (optimum, 25-30 °C), at pH 7.