Publications by authors named "Hyuk-Jin Moon"
The following study was conducted to generate a transgenic Sf9 cell line for rapid and easy virus quantification in the baculovirus expression system (BES). The hr3 (homologous region 3) and and promoters were used as the expression structures to induce rapid and intense expression of the enhanced green fluorescent protein gene in cells in response to viral infection. Of 20 transgenic Sf9 cell lines generated using the piggyBac system, the cell line that showed the highest fluorescence expression in the shortest time in response to viral infection was selected and named Sf9-QE.
View Article and Find Full Text PDFArticle Synopsis
- The study aimed to enhance the production of virus-like particles (VLPs) for enterovirus 71 (EV71), which causes hand, foot, and mouth disease (HFMD).
- Researchers focused on optimizing the expression levels of the P1 precursor protein and the 3CD protein necessary for cleaving P1, adjusting factors like promoter strength.
- Findings showed that using a specific weakened promoter led to effective P1 and 3CD expression, resulting in high structural protein production and improved VLP formation for HFMD.
Background: An oral glucose tolerance test (OGTT) is the current method used for screening and diagnosis of gestational diabetes mellitus (GDM). OGTT is a relatively complicated procedure and is expensive. Thus, new strategies that do not require fasting or more than a single blood draw may improve the diagnosis of GDM and increase the rate of GDM testing.
View Article and Find Full Text PDF