Publications by authors named "Hyoeun Lee"
Article Synopsis
- - The study focused on estimating pharmacokinetic (PK) parameters for an obese hemodialysis patient using gentamicin, highlighting how obesity affects drug clearance (CL).
- - A 53-year-old obese woman receiving hemodialysis was analyzed, showing a significant reduction in her non-HD gentamicin clearance compared to population averages, with her creatinine clearance (CrCL) indicating potential inaccuracies in renal function assessment.
- - Results from different modeling software (NONMEM and Monolix) underscored the necessity for individualized PK evaluations, particularly for obese patients undergoing hemodialysis, to accurately determine drug clearance impacts.
Article Synopsis
- Cryopreservation at -196 °C is a key method for long-term storage of non-orthodox seeds and wild flora, though its implementation faces challenges like poor in vitro propagation techniques and a lack of standard protocols.
- This study created a systematic droplet-vitrification cryopreservation method specifically for shoot tips, using a two-step sucrose preculture and specialized cryoprotective solutions.
- The results showed that using liquid overlay during regrowth significantly improved plantlet development from cryopreserved samples, achieving an 80% regeneration rate—23% higher than without the overlay.
Article Synopsis
- - Cryopreservation using liquid nitrogen is a method for preserving endangered plants with seeds that can't be stored in a traditional manner, employing a droplet-vitrification protocol that involves multiple steps including osmoprotection and specific regrowth conditions.
- - The study focused on analyzing organic acids produced during the cryopreservation process to understand their relationship with plant metabolism and stress, using gas chromatography to analyze 14 different acids.
- - Results showed that the success of shoot tip regeneration was greatest under optimal conditions, with decreasing percentages of successful regrowth linked to deviations in the protocol, emphasizing the importance of maintaining proper conditions for cryopreservation.
This study aimed to investigate the effects of different injection sites, including dorsal, cheek, and pectoral fin muscles, on the pharmacological properties of amoxicillin (AMOX) in olive flounder () after a single intramuscular (IM) injection of 40 mg/kg. The AMOX concentration was measured using high-performance liquid chromatography-tandem mass spectrometry, followed by a non-compartmental model analysis. The peak serum concentrations (C) achieved 3 h after dorsal, cheek, and pectoral fin IM injections were 202.
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- Cryopreservation, a method that involves storing biological materials at extremely low temperatures, is increasingly used for conserving non-orthodox seeds and plants, but there is still a lack of universal protocols for its application.
- This study developed a detailed droplet-vitrification procedure for cryopreserving chrysanthemum shoot tips, including specific steps for preculture, osmoprotection, and cryoprotection.
- The successful pilot cryobanking of 154 chrysanthemum accessions resulted in a 74.8% regeneration rate post-cryopreservation, showcasing its potential for long-term conservation of the Asteraceae family germplasm.
Cryopreservation is a unique option for the long-term conservation of threatened plant species with non-orthodox or limitedly available seeds. However, the wide application of cryopreservation for the protection of wild flora is hampered by some reasons: limits of source material available, difficulties in in vitro propagation, needs to re-optimize protocol steps for new species, etc. In this study, using an endemic and endangered Korean species, , we investigated subculture medium and supplements on in vitro growth of donor plants: medium strength, gelling agents, liquid overlay, plant hormones, and activated charcoal.
View Article and Find Full Text PDFIn the Republic of Korea, (EHP) was first isolated from Pacific whiteleg shrimp in April 2020; however, there are no existing reports of EHP infection in other shrimp or prawns. Here, we aimed to investigate EHP infection and its prevalence in giant freshwater prawn farms in the Republic of Korea. We tested prawns from 22 farms for EHP infection, and samples from eight farms showed positive EHP infection results in 2021.
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- This study explores better methods for ex situ conservation of the Pursh plant through in vitro seed germination and nodal explant multiplication.
- It found that a liquid medium overlay boosted shoot and root growth, while activated charcoal and certain growth regulators harmed it.
- Successful cryopreservation of shoot tips was achieved using a detailed step-by-step method, improving regeneration rates by optimizing growth conditions and reducing stress from chemicals and nutrients.
Article Synopsis
- Self-propagating autocatalytic reactions of proteases have not been used in affinity-based biosensors due to limited reactions and issues with self-activation of proenzymes.
- The study introduces a mutant trypsinogen that minimizes self-activation and, when activated by trypsin, facilitates high signal amplification, leading to very low detection limits for prostate-specific antigen (PSA).
- The process involves autocatalytic activation, proteolytic cleavage, and redox cycling, resulting in a PSA detection limit of approximately 7 pg/mL, significantly better than the limit achieved using trypsin alone.
Cryopreservation provides a secure long-term conservation option for rare and endangered plant species with non-orthodox or limitedly available seeds. Wide application of cryopreservation to biobank wild flora is hampered by the need to re-optimize nearly all protocol steps for every new species. We applied a systematic approach to simplify optimization of a multi-stage droplet-vitrification method for the endangered wetland Korean species, .
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- In the adult hippocampus, synapses are continuously formed and removed, but the role and regulation of this synapse elimination remain largely unclear.
- The study reveals that astrocytes, a type of glial cell, are crucial for eliminating excitatory synapses through a receptor called MEGF10, influencing synaptic connectivity and plasticity in the CA1 region of the hippocampus.
- Mice lacking the MEGF10 receptor in astrocytes show a buildup of functionally impaired excitatory synapses, leading to defective long-term synaptic plasticity and challenges in memory formation, highlighting the importance of astrocytes in maintaining cognitive function.
Understanding brain function-related neural circuit connectivity is essential for investigating how cognitive functions are decoded in neural circuits. Trans-synaptic viral vectors are useful for identifying neural synaptic connectivity because of their ability to be transferred from transduced cells to synaptically connected cells. However, concurrent labeling of multisynaptic inputs to postsynaptic neurons is impossible with currently available trans-synaptic viral vectors.
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- * Researchers used a defective form of KIF5A, a motor protein, to show that its absence lowers PSD-95 levels in dendrites, indicating KIF5's role in transporting PSD-95.
- * The study also found a dose-dependent relationship between the Staufen protein and the association of PSD-95 with KIF5A, suggesting Staufen may help regulate this transport process.
Article Synopsis
- The study aimed to identify factors associated with electrocardiographic left ventricular hypertrophy (ECG-LVH) among normotensive individuals without a history of hypertension.
- It analyzed data from participants at Hallym University Sacred Heart Hospital, comparing those with ECG-LVH to healthy controls.
- Results indicated that factors like male sex, age, and systolic blood pressure were linked to ECG-LVH, with additional differences in risk profiles between men and women, particularly regarding exercise habits.
Article Synopsis
- The anterior cingulate cortex (ACC) is linked to fear behavior control, particularly in response to innate threats, yet the mechanisms remain unclear.
- Research shows that the ACC influences the basolateral nucleus of the amygdala (BLA), affecting innate fear responses to predator odors in mice.
- Optogenetic techniques reveal that inactivating the ACC increases freezing behavior to fox urine, while stimulating it reduces both innate and learned freezing responses.
Article Synopsis
- The study investigates the link between how soon a smoker has their first cigarette after waking and the risk of developing obstructive respiratory issues, such as chronic obstructive pulmonary disease (COPD).
- Researchers analyzed data from 392 current smokers, distinguishing those who smoked within 30 minutes of waking (early group) from those who waited longer (late group).
- Findings showed that smokers who had their first cigarette within 30 minutes had over twice the risk of obstructive pulmonary impairment compared to those who waited longer, suggesting this timing could be a key factor in assessing risk for respiratory diseases.
Article Synopsis
- This study investigated the antioxidant effects of 70% ethanol extract from GNE on cell lines exposed to hydrogen peroxide-induced damage.
- The results demonstrated that GNE effectively scavenged free radicals and contained high levels of polyphenols and flavonoids, which contributed to its protective effects against oxidative stress.
- These findings suggest that GNE could be beneficial in preventing diseases related to oxidative stress, such as Alzheimer's, respiratory inflammation, and chronic kidney diseases.