Rapid Real-Time Polymerase Chain Reaction for Serotyping Based on Novel Unique Gene Markers by Pangenome Analysis.

An accurate diagnostic method for serovars is fundamental to preventing the spread of associated diseases. A diagnostic polymerase chain reaction (PCR)-based method has proven to be an effective tool for detecting pathogenic bacteria. However, the gene markers currently used in real-time PCR to detect serovars have low specificity and are developed for only a few serovars.

Direct loop-mediated isothermal amplification (LAMP) assay for rapid on-site detection of Bifidobacterium longum subspecies longum, infantis, and suis in probiotic products.

As interest in probiotics increases, the need for accurate description of probiotic compositions present in commercial products is also increasing. Since Bifidobacterium longum used as probiotics is labeled at species or subspecies levels, a detection method for distinguishing B. longum subsp.

Development of a Genoserotyping Method for Infantis Detection on the Basis of Pangenome Analysis.

In recent years, Infantis has become a predominant serovariant in clinical and poultry isolates, thereby imposing a substantial economic burden on both public health and the livestock industry. With the aim of coping with the steep increase in serovar Infantis prevalence, a polymerase chain reaction (PCR)-based rapid and accurate diagnostic assay was developed in this study through pangenome profiling of 60 serovars. A gene marker, SIN_02055, was identified, which is present in the .

Development of Real-Time PCR Assay to Specifically Detect 22 Species and Subspecies Using Comparative Genomics.

species are used as probiotics to provide beneficial effects to humans. These effects are specific to some species or subspecies of . However, some species or subspecies are not distinguished because similarity of 16S rRNA and housekeeping gene sequences within species is very high.

Novel specific peaks for differentiating the Lactobacillus plantarum group using matrix-assisted laser desorption ionization time-of-flight mass spectrometry.

Identifying the Lactobacillus plantarum group using conventional taxonomic methods such as biochemical analysis and 16S rRNA gene sequencing is inaccurate, expensive, and time-consuming. In this study, for the first time, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry was used to identify the L. plantarum group and develop a classification method for species level differentiation with specific peaks based on mass spectra.