Psychrotrophic Bacteria Threatening the Safety of Animal-Derived Foods: Characteristics, Contamination, and Control Strategies.

Animal-derived foods, such as meat and dairy products, are prone to spoilage by psychrotrophic bacteria due to their high-water activity and nutritional value. These bacteria can grow at refrigerated temperatures, posing significant concerns for food safety and quality. Psychrotrophic bacteria, including , , and , not only spoil food but can also produce heat-resistant enzymes and toxins, posing health risks.

Quantitative risk assessment of foodborne illness by estimating cooking effect on eggs from retail markets.

In this study, we performed a quantitative microbial risk assessment (QMRA) of through intake of egg consumption after cooking (dry-heat, moist-heat, and raw consumption). Egg samples (n = 201) from retail markets were analyzed for the presence of . In addition, temperature and time were investigated during egg transit, storage, and display.

Isolation of Debaryomyces hansenii and selection of an optimal strain to improve the quality of low-grade beef rump (middle gluteal) during dry aging.

Objective: The objective of this study was to evaluate the effect of Debaryomyces hansenii isolated from dry-aged beef on the tenderness and flavor attributes of low-grade beef during dry aging.

Methods: Five D. hansenii strains were isolated from dry-aged beef samples.

Risk Assessment in Poultry Meat from Farm to Consumer in Korea.

This study predicted outbreak risk from eating cooked poultry in various methods. The incidence of in poultry meat and the environment from farm to home for consumption was investigated. To develop the predictive models, growth data were collected at 4-25 °C during storage and fitted with the Baranyi model.

Reconstruction of mandibular defects in osteoradionecrosis and medication-related osteonecrosis of the jaw using fibula free flap and management of postoperative wound infections.

Background: Complications from osteoradionecrosis (ORN) and medication-related osteonecrosis of the jaw (MRONJ) include oro-cutaneous fistulas, necrotic bone exposure, soft-tissue defects, and pathologic fractures. The fibula free flap (FFF) is a common free flap method used to reconstruct the mandible in severe cases. Recently, we have used the FFF successfully for the reconstruction of ORN and MRONJ mandibular defects.

Isolation of Bacillus cereus from Soft Soybean Curd and the Kinetic Behavior of B. cereus Isolates at Changing Temperatures.

Abstract: In this study, Bacillus cereus was isolated from soft soybean curds, and a dynamic model was developed to describe the kinetic behavior of these isolates during transfer and storage. B. cereus isolates recovered from soft soybean curds were inoculated into soft soybean curd, and the levels were determined during storage at 10 to 30°C.

Erratum to: Description of Kinetic Behavior of Pathogenic in Cooked Pig Trotters under Dynamic Storage Conditions Using Mathematical Equations.

[This corrects the article DOI: 10.5851/kosfa.2020.

High Prevalence of in Smoked Duck: Antibiotic and Heat Resistance, Virulence, and Genetics of the Isolates.

This study aimed at determining the genetic and virulence characteristics of the from smoked ducks. was isolated by plating, and the isolated colonies were identified by PCR. All the obtained seven isolates possessed the virulence genes (A, B, B, and A) and a 385 bp A amplicon.

SMFM2017-NK4 Isolated from Kimchi Can Prevent Obesity by Inhibiting Fat Accumulation.

This study evaluated the anti-obesity effects of lactic acid bacteria. Thirty-one lactic acid bacteria were examined in vitro for their ability to inhibit α-glucosidase activity, lipase activity, and 3T3-L1 cell differentiation. Four selected lactic acid bacteria were administered to obese C57BL/6J mice models for 8 weeks.

Development of a Selective Agar for Improving Campylobacter jejuni Detection in Food.

Background: Campylobacter jejuni is a major gastroenteritis-causing foodborne pathogen. However, it is difficult to isolate when competing bacteria or cold-damaged cells are present.

Objective: Herein, a medium (Campylobacter selective agar, CSA) was developed and supplemented with catalase, L-serine, L-cysteine, and quercetin for the selective detection of C.

Contamination of in soy sauce, and quantitative microbial risk assessment for through soy sauce consumption.

The objective of this study was to conduct QMRA (quantitative microbial risk assessment) of through soy sauce consumption. Four hundred and ninety soy sauce samples from markets were analyzed to detect . Temperature and time were also measured during transportation and display of soy sauce.

Antimicrobial activity of fermented Maillard reaction products, novel milk-derived material, made by whey protein and Lactobacillus rhamnosus and Lactobacillus gasseri on Clostridium perfringens.

Objective: The objective of this study was to evaluate the antimicrobial effects of fermented Maillard reaction products made by milk proteins (FMRPs) on Clostridium perfringens (C. perfringens), and to elucidate antimicrobial modes of FMRPs on the bacteria, using physiological and morphological analyses.

Methods: Antimicrobial effects of FMRPs (whey protein plus galactose fermented by Lactobacillus rhamnosus [L.

Identification of Pathogenic Variations in Seafood Vibrio parahaemolyticus Isolates by Comparing Genome Sequences.

Abstract: An investigation of the pathogenic characteristics of isolates of Vibrio parahaemolyticus was conducted by identifying the pathogenic tdh gene and then performing adherence and cytotoxicity assays. Genome sequences of the seafood isolates were analyzed using the Illumina HiSeq 2500 platform. The isolated strains were then mapped by comparing the genomes to the reference genome, and variations in the nucleotide sequences and amino acids were identified with the CLC Genomics Workbench program.

Synthesis of nitrogen-doped carbon nanodots to destroy bacteria competing with Campylobacter jejuni in enrichment medium, and development of a monoclonal antibody to detect C. jejuni after enrichment.

The objective of this study was to develop a method with improved sensitivity for Campylobacter jejuni detection in foods. Nitrogen-doped carbon nanodots (N-CNDs) were synthesized and added to an enrichment medium (Bolton broth) at a concentration of 10 mg/mL. A light-emitting diode (LED) at a wavelength of 425 nm was used to irradiate the N-CNDs-supplemented enrichment medium to induce an exothermic reaction for 1 h.

Description of Kinetic Behavior of Pathogenic in Cooked Pig Trotters under Dynamic Storage Conditions Using Mathematical Equations.

A dynamic model was developed to predict the cell counts in pig trotters at changing temperatures. Five-strain mixture of pathogenic at 4 Log CFU/g were inoculated to cooked pig trotter samples. The samples were stored at 10°C, 20°C, and 25°C.

Intestinal Can Cause Liver Injury through the Occurrence of Inflammation and Damage to Hepatocytes.

This study investigated if intestinal (CD) causes liver injury. Four-week-old male C3H/HeN mice were treated with phosphate-buffered solution (control), CD, diethylnitrosamine (DEN) to induce liver injury with PBS (DEN+PBS), and DEN with CD (DEN+CD) for nine weeks. After sacrifice, livers and mesenteric lymph nodes (MLNs) were removed and bacterial translocation, transcriptomes, and proteins were analysed.

Influence of milk microbiota on survival during cheese ripening.

This study aimed to compare the three strains of survival in raw milk cheese and pasteurized milk cheese and to suggest the effect of milk microbiota on survival. cell counts decreased in all cheese as ripening time increased, and the survival rate was different for the strains of . Furthermore, survived longer in raw milk cheese than in pasteurized milk cheese.

Anti-Inflammatory Effect of a Peptide Derived from the Synbiotics, Fermented with , on Inflammatory Bowel Disease.

The objective of this study was to evaluate the effects of peptides derived from synbiotics on improving inflammatory bowel disease (IBD). Five-week-old male C57BL/6 mice were administered with dextran sulfate sodium (DSS) via drinking water for seven days to induce IBD (IBD group). The mice in the IBD group were orally administered with PBS (IBD-PBS-positive control), 505 (IBD-Pro), fermented powder of CT extract with 505 (IBD-Syn), -casein: LSQSKVLPVPQKAVPYPQRDMP (IBD-Pep 1), or -casein: VYQHQKAMKPWIQPKTKVIPYVRYL (IBD-Pep 2) (both peptides are present in the synbiotics) for four more days while inducing IBD.

Quantitative microbial risk assessment for foodborne illness following consumption of kimchi in South Korea.

The purpose of this study was to conduct a risk assessment for foodborne illness via kimchi consumption in South Korea. Prevalence of in kimchi, kimchi consumption amount and frequency, and distribution conditions (time and temperature) from manufacture to the home were determined. initial contamination level was estimated using Beta distribution [Beta (6, 79)].

The role of Pseudomonas aeruginosa DesB in pathogen-host interaction.

Pseudomonas aeruginosa, commonly found in environments, can cause chronic lung disease in immunocompromised patients. In previous study, an aerobic desaturase (DesB) in P. aeruginosa exerted considerable effects on virulence factor production.

Akkermansia muciniphila Prevents Fatty Liver Disease, Decreases Serum Triglycerides, and Maintains Gut Homeostasis.

The objective of this study was to elucidate the effect of intestinal bacteria on fatty liver disease. Five-week-old C57BL/6N mice were administered either phosphate-buffered saline (PBS; control) or at 10 to 10 CFU/ml, and were fed either a 45% fat diet (high-fat diet [HFD]) or a 10% fat diet (normal diet [ND]) for 10 weeks. After 10 weeks, the mice were euthanized, and blood and tissue samples, including adipose tissue, cecum, liver, and brain, were immediately collected.

Asymptomatic Inhabitation in Intestine Can Cause Inflammation, Apoptosis, and Disorders in Brain.

(CP) is a foodborne pathogen. The bacterium can also inhabit human gut without symptoms of foodborne illness. However, the clinical symptoms of long-term inhabitation have not been known yet.

Combined Enrichment and Quantitative Polymerase Chain Reaction to Improve Sensitivity and Reduce Time of Detection of in Mushrooms.

This study evaluated a combined method for the detection of in mushrooms, involving enrichment and quantitative real-time polymerase chain reaction (qPCR), to improve sensitivity and reduce detection time. The growth was evaluated in enrichment broth (LEB) with modified carbon and nitrogen sources, increasing sodium concentrations, and added micronutrients. Primers targeting the (1 2), (1-6), and (1-4) genes were developed and their sensitivity and specificity were evaluated.

Identification of Microorganisms Associated with the Quality Improvement of Dry-Aged Beef Through Microbiome Analysis and DNA Sequencing, and Evaluation of Their Effects on Beef Quality.

The objective of this study was to isolate and identify the microorganisms, especially yeasts and molds, related to the improvement of beef quality during dry-aging of beef through microbiome analysis, and to examine the possibility of using them as starter culture strains to improve the efficiency of dry-aging beef production. Beef sirloins were dry-aged for 28 days using different wind speeds (0, 2.5, and 5 m/s) at 1 to 3 °C and 75% relative humidity, and microbial compositions were confirmed by microbiome analysis.

Quantitative Microbial Risk Assessment for in Ground Meat Products in Korea.

This study evaluated risk in ground meat products. The prevalence in ground meat products was investigated. To develop the predictive model, survival data of were collected at 4°C-30°C during storage, and the data were fitted using the Weibull model.