Oligomeric HIV-1 Integrase Structures Reveal Functional Plasticity for Intasome Assembly and RNA Binding.

Integrase (IN) performs dual essential roles during HIV-1 replication. During ingress, IN functions within an oligomeric "intasome" assembly to catalyze viral DNA integration into host chromatin. During late stages of infection, tetrameric IN binds viral RNA and orchestrates the condensation of ribonucleoprotein complexes into the capsid core.

Effect of superspeed sintering on translucency, opalescence, microstructure, and phase fraction of multilayered 4 mol% yttria-stabilized tetragonal zirconia polycrystal and 6 mol% yttria-stabilized partially stabilized zirconia ceramics.

Statement Of Problem: The optical properties of recently developed multilayer zirconia have mainly been studied for the effects of conventional sintering and speed sintering but not as much for the effect of superspeed sintering. As superspeed sintering protocols typically require a higher sintering temperature and higher heating and cooling rates than speed- and conventional sintering protocols, the optical properties of superspeed sintered zirconia may be affected differently.

Purpose: The purpose of this in vitro study was to investigate the effect of superspeed sintering on the optical properties, microstructure, and phase fraction of multilayered 4 mol% yttria-stabilized (4Y-) and 6 mol% yttria-stabilized (6Y-) zirconia.

Bacterial Reverse Mutation Test of Verbenalin.

Objectives: Verbenalin is a compound found in herbs such as and . This study investigated whether verbenalin is safe by analyzing its mutagenicity.

Methods: To examine the mutagenic potential of verbenalin, a bacterial reverse mutation test (Ames test) was conducted with and strains.

Effect of Cooling Rate on Mechanical Properties, Translucency, Opalescence, and Light Transmission Properties of Monolithic 4Y-TZP during Glazing.

A standard cooling rate has not been established for glazing; therefore, the effects of the cooling rate on the properties of zirconia need to be evaluated to predict outcomes in clinical practice. 4Y-TZP glazed at three different cooling rates was analyzed to estimate the effect of cooling rate during glazing on the mechanical and optical properties. Hardness tests, field-emission scanning electron microscopy analysis, X-ray diffraction analysis, flexural strength measurement, and optical property evaluations were performed.

Effect of cooling rate on hardness and microstructure of Pd-Ag-In-Sn-Ga alloy during porcelain firing simulation.

This study examined the effect of the cooling rate on the hardness and its effect on the microstructure during porcelain firing simulation of a Pd-Ag-In-Sn-Ga metal-ceramic alloy. In practice, after each firing step for porcelain bonding, the prosthesis is cooled to room temperature before proceeding to the next firing step. The cooling step is known to allow the hardness of the metal substructure to increase.

Effect of ice quenching after oxidation with or without vacuum on the hardness of Pd-Ag-Au-In alloy during porcelain firing simulation.

This study examined the effect of ice-quenching after oxidation treatment with or without vacuum, on the change in the hardness of a Pd-Ag-Au-In metal-ceramic alloy during porcelain-firing simulation. The aim of the present study is to determine whether ice quenching after oxidation renders a Pd-Ag metal-ceramic alloy soft enough for easy processing without the need for an additional solution treatment and whether it affects the final hardness of the Pd-Ag metal-ceramic alloy after porcelain firing simulation. The oxidation treatment resulted in the as-cast specimen becoming homogenized, which was progressed further by vacuuming.

Effect of ice-quenching after oxidation treatment on hardening of a Pd-Cu-Ga-Zn alloy for bonding porcelain.

This study examined the effect of ice-quenching after oxidation treatment on hardness change of a Pd-Cu-Ga-Zn metal-ceramic alloy during porcelain firing simulation. Although not statistically significant, the alloy was softened slightly during porcelain firing simulation with conventional slow cooling rate. On the other hand, the hardness increased significantly by ice-quenching instead of the slow cooling after oxidation (p<0.

Effect of ice-quenching on the change in hardness of a Pd-Au-Zn alloy during porcelain firing simulation.

This study examined the effect of ice-quenching after degassing on the change in hardness of a Pd-Au-Zn alloy during porcelain firing simulations. By ice-quenching after degassing, the specimens were softened due to homogenization without the need for an additional softening heat treatment. The lowered hardness by ice-quenching after degassing was recovered greatly from the first stage of porcelain firing process by controlling the cooling rate.

IL-1 receptor antagonist (IL-1Ra)-Fc ameliorate autoimmune arthritis by regulation of the Th17 cells/Treg balance and arthrogenic cytokine activation.

Introduction: IL-1β signalling has a critical role in the pathogenesis of various types of inflammatory arthritis including rheumatoid arthritis (RA). We aimed to investigate the therapeutic effects of human IL-1 receptor antagonist with Fc fragment (hIL-1Ra-Fc) on autoimmune arthritis and to identify the possible mechanisms by which hIL-1RA-Fc exerts anti-arthritic effects in a murine model of RA and patients with rheumatoid arthritis.

Methods: Collagen-induced arthritis (CIA) murine model was established in DBA/1J mice.

Crystallization and X-ray diffraction data collection of topoisomerase IV ParE subunit from Xanthomonas oryzae pv. oryzae.

Topoisomerase IV is involved in topological changes in the bacterial genome using the free energy from ATP hydrolysis. Its functions are the decatenation of daughter chromosomes following replication by DNA relaxation and double-strand DNA breakage. In this study, the N-terminal fragment of the topoisomerase IV ParE subunit from Xanthomonas oryzae pv.

The crystal structure of ferritin from Helicobacter pylori reveals unusual conformational changes for iron uptake.

The crystal structure of recombinant ferritin from Helicobacter pylori has been determined in its apo, low-iron-bound, intermediate, and high-iron-bound states. Similar to other members of the ferritin family, the bacterial ferritin assembles as a spherical protein shell of 24 subunits, each of which folds into a four-alpha-helix bundle. Significant conformational changes were observed at the BC loop and the entrance of the 4-fold symmetry channel in the intermediate and high-iron-bound states, whereas no change was found in the apo and low-iron-bound states.

Induction of mucosal and systemic immune responses following oral immunization of mice with Lactococcus lactis expressing human papillomavirus type 16 L1.

Human papillomavirus type 16 L1 (HPV16 L1) has shown considerable promise as a parenteral vaccine for prevention of cervical cancers. Here, we report the possibility of oral vaccination for HPV16 L1 using Lactococcus lactis (L. lactis) as a live vector.

Insight into autoproteolytic activation from the structure of cephalosporin acylase: a protein with two proteolytic chemistries.

Cephalosporin acylase (CA), a member of the N-terminal nucleophile hydrolase family, is activated through sequential primary and secondary autoproteolytic reactions with the release of a pro segment. We have determined crystal structures of four CA mutants. Two mutants are trapped after the primary cleavage, and the other two undergo secondary cleavage slowly.