Publications by authors named "Hyacinta Majekova"
Antimicrobial and immunomodulatory potential of various Lactobacillus reuteri strains is closely connected to their metabolite production profile under given cultivation conditions. We determined the in vitro production of antimicrobial substances such as organic acids, ethanol, and reuterin by four strains of L. reuteri (L.
View Article and Find Full Text PDFBefore use in practice, it is necessary to precisely identify and characterize a new probiotic candidate. Eight animal lactobacilli and collection strain Lactobacillus reuteri CCM 3625 were studied from the point of saccharide fermentation profiles, bile salt resistance, antibiogram profiles, and influence of bile on sensitivity to antibiotics. Studied lactobacilli differed in their sugar fermentation ability determined by API 50CHL and their identification based on these profiles did not correspond with molecular-biological one in most cases.
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- * The ITS-PCR method and protein analysis techniques like SDS-PAGE and MALDI-TOF successfully differentiated the strains, while ITS-PCR/RFLP and ERIC-PCR were found to be ineffective.
- * Consistent clustering was observed in most strains across different methods, although MALDI-TOF MS separated goatling strains from the lamb isolate, indicating variability in strain grouping.