Multiplex TaqMan qPCR Assay for Detection, Identification, and Quantification of Three Species.

White mold (or Sclerotinia stem rot), caused by species, is a major air, soil, or seed-transmitted disease affecting numerous crops and wild plants. Microscopic or culture-based methods currently available for their detection and identification are time-consuming, laborious, and often erroneous. Therefore, we developed a multiplex quantitative PCR (qPCR) assay for the discrimination, detection, and quantification of DNA collected from each of the three economically relevant species, namely, , , and .