Publications by authors named "Huynh Kim Khanh Ta"

Human interleukin-3 (IL3) is a multifunctional cytokine essential for both clinical and biomedical research endeavors. However, its production in Escherichia coli has historically been challenging due to its aggregation into inclusion bodies, requiring intricate solubilization and refolding procedures. This study introduces an innovative approach employing two chaperone proteins, maltose binding protein (MBP) and protein disulfide isomerase b'a' domain (PDIb'a'), as N-terminal fusion tags.

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Article Synopsis
  • Recombinant immunotoxins (RITs) are engineered fusion proteins that combine a targeting domain with a toxin, allowing for precise cancer therapy aimed at overexpressed proteins like mesothelin found in certain tumors.
  • Researchers developed RITs targeting mesothelin by selecting specific nanobodies and optimizing them for better expression and purification, achieving high solubility and purity in the process.
  • The most effective RIT, Meso(Nb2)-PE24B, demonstrated strong cytotoxicity against gastric cancer cell lines and selective binding to human mesothelin, suggesting it could be a valuable candidate for targeted cancer treatment in future studies.
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Human epidermal growth factor receptor 2 (HER-2) is overexpressed in many malignant tumors. The anti-HER2 antibody trastuzumab has been approved for treating HER2-positive early and metastatic breast cancers. exotoxin A (PE), a bacterial toxin of , consists of an A-domain with enzymatic activity and a B-domain with cell binding activity.

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Human stem-cell factor (hSCF) stimulates the survival, proliferation, and differentiation of hematopoietic cells by binding to the c-Kit receptor. Various applications of hSCF require the efficient and reliable production of hSCF. hSCF exists in three forms: as two membrane-spanning proteins hSCF248 and hSCF229 and truncated soluble -terminal protein hSCF164.

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Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a member of the colony-stimulating factor (CSF) family, which functions to enhance the proliferation and differentiation of hematopoietic stem cells and other hematopoietic lineages such as neutrophils, dendritic cells, or macrophages. These proteins have thus generated considerable interest in clinical therapy research. A current obstacle to the prokaryotic production of human GM-CSF (hGM-CSF) is its low solubility when overexpressed and subsequent complex refolding processes.

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