Publications by authors named "Huu Hien Huynh"
Article Synopsis
- The study develops a high-throughput assay to measure peptides from type III procollagen in human serum, addressing limitations of existing immunoassays used for growth hormone detection.
- The new method utilizes a combination of immunoaffinity enrichment and ultra-high-performance liquid chromatography-tandem mass spectrometry, resulting in good accuracy and precision for quantifying the peptides.
- While this method shows a decent correlation to a standard immunoassay, significant bias indicates a need for standardized calibrators to improve result consistency across different laboratories.
Article Synopsis
- - Many peptide hormones adopt an α-helix structure when they bind to their receptors, and developing sensitive detection methods could improve disease management.
- - Researchers are using advanced techniques, like parametric generation and deep learning, to design proteins that can effectively interact with helical peptides, which has been a challenging task.
- - The study introduces RFdiffusion, an innovative approach that allows for the creation of high-affinity binders for flexible peptide targets, enabling enhanced detection methods such as mass spectrometry and biosensor development.
Background: APOE genotype is associated with Alzheimer disease. Thus, the concentration of apolipoprotein E (apoE) isoforms in cerebrospinal fluid (CSF) could be altered in dementia. However, conflicting results have been obtained in different studies.
View Article and Find Full Text PDFBackground: The development of analytical approaches to help reduce the risk of growth hormone (GH) doping is important to fair competition and the health of athletes. However, the reliable detection of GH use remains challenging. The identification of novel biomarkers of GH administration could lead to a better understanding of the physiological response to GH, more sensitive detection of the illicit use of GH in sport, and better management of patients treated for GH disorders.
View Article and Find Full Text PDFProcalcitonin (PCT) is a widely used biomarker for rapid sepsis diagnosis and antibiotic stewardship. Variability of results in commercial assays has highlighted the need for standardization of PCT measurements. An antibody-free candidate reference measurement procedure (RMP) based on the isotope dilution mass spectrometry and protein calibration approach was developed and validated to quantify PCT in human serum.
View Article and Find Full Text PDFSepsis represents a global health priority because of its high mortality and morbidity. The key to improving prognosis remains an early diagnosis to initiate appropriate antibiotic treatment. Procalcitonin (PCT) is a recognized biomarker for the early indication of bacterial infections and a valuable tool to guide and individualize antibiotic treatment.
View Article and Find Full Text PDFThe quantification of low abundant proteins in complex matrices by liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) remains challenging. A measurement procedure based on optimized antibody-free sample preparation and isotope dilution coupled to LC-MS/MS was developed to quantify procalcitonin (PCT) in human serum at sub-microgram per liter level. A combination of sodium deoxycholate-assisted protein precipitation with acetonitrile, solid-phase extraction, and trypsin digestion assisted with Tween-20 enhanced the method sensitivity.
View Article and Find Full Text PDFProcalcitonin (PCT) is an important biomarker for sepsis diagnosis and management. To date, there is no higher-order reference measurement procedure (RMP) and certified reference material to achieve global standardization of results and results traceability to the SI units. Although efforts have been made to harmonize PCT results, a number of comparison studies and external quality assessment (EQA) schemes show conflicting results regarding results comparability and to date, equivalence of PCT results across the assays remains questionable in absence of studies relying on commutable EQA materials.
View Article and Find Full Text PDFBackground: A simple, rapid, and sensitive liquid chromatography coupled with tandem mass spectrometry method has been developed and validated for the quantification of ruxolitinib, olaparib, vismodegib, and pazopanib in human plasma.
Methods: After a simple protein precipitation of plasma samples, the chromatographic separation was performed using an ultraperformance liquid chromatography system coupled with mass tandem spectrometry in a positive ionization mode. The mobile phase consisted of a gradient elution of 10-mmol/L formate ammonium buffer containing 0.