Single and combined effects of metal-based fungicides on Eisenia andrei in different scenarios of climatic change.

This study evaluated the ecotoxicity of metal-based fungicides under the current scenarios of global climatic change (20 °C and 25 °C) and moisture content (30% and 50%) in single and binary mixtures of copper oxychloride (CuOx) [200, 500 and 1000 mg/kg] and mancozeb (MnZn) [44, 850 and 1250 mg/kg]. Endpoints assessed included mortality, changes in biomass, avoidance behaviour, and reproduction utilising standardised protocols (ISO and OECD). The changes in biomass and mortality tests lasted 28 days, followed by a 28-day reproduction test and a two-day avoidance test.