Atypical microorganisms in expressed prostatic secretion from patients with chronic prostatitis/chronic pelvic pain syndrome: microbiological results from a case-control study.

Objectives: To investigate the microorganisms in expressed prostatic secretions (EPS) of both chronic prostatitis patients and normal young male adults and to determine which microorganisms are associated with the degree of intraprostatic inflammation.

Methods: Specific polymerase chain reaction (PCR) technology was performed to confirm Neisseria gonorrhoeae, Mycobacterium tuberculosis, Mycoplasma and Chlamydia trachomatis as well as human papilloma virus (HPV), herpes simplex virus type 2 (HSV-2) and cytomegalovirus (CMV). Universal primer PCR technology was carried out to detect 16S bacteria rDNA, followed by cloning and sequencing of the entire 16S rDNA product.

Association of MIF-173 gene polymorphism with inflammatory bowel disease in Chinese Han population.

Aim: To study whether macrophage migration inhibitory factor (MIF)-173 gene polymorphism correlates with inflammatory bowel disease (IBD) in Chinese Han population.

Methods: MIF-173 single nucleotide polymorphism (SNP) was genotyped by tetra-primer amplification refractory mutation system (ARMS) and restriction fragment length polymorphisms (RFLP)-PCR in 142 healthy subjects and 98 patients with inflammatory bowel disease (IBD).

Results: There were no discrepancies between the results obtained by tetra-primer ARMS and RFLP-PCR.