A single-cell atlas of the midgut during West Nile virus infection.

The mosquito midgut functions as a key interface between pathogen and vector. However, studies of midgut physiology and virus infection dynamics are scarce, and in - an extremely efficient vector of West Nile virus (WNV) - nonexistent. We performed single-cell RNA sequencing on midguts, defined multiple cell types, and determined whether specific cell types are more permissive to WNV infection.