Systematic Optimization of Protein Secretory Pathways in to Increase Expression of Hepatitis B Small Antigen.

Hepatitis B is a major disease that chronically infects millions of people in the world, especially in developing countries. Currently, one of the effective vaccines to prevent Hepatitis B is the Hepatitis B Small Antigen (HBsAg), which is mainly produced by the recombinant yeast . In order to bring down the price, which is still too high for people in developing countries to afford, it is important to understand key cellular processes that limit protein expression.