Comprehensive evaluation and practical guideline of gating methods for high-dimensional cytometry data: manual gating, unsupervised clustering, and auto-gating.

Cytometry is an advanced technique for simultaneously identifying and quantifying many cell surface and intracellular proteins at a single-cell resolution. Analyzing high-dimensional cytometry data involves identifying and quantifying cell populations based on their marker expressions. This study provided a quantitative review and comparison of various ways to phenotype cellular populations within the cytometry data, including manual gating, unsupervised clustering, and supervised auto-gating.

Adaptively Integrative Association between Multivariate Phenotypes and Transcriptomic Data for Complex Diseases.

Phenotype-gene association studies can uncover disease mechanisms for translational research. Association with multiple phenotypes or clinical variables in complex diseases has the advantage of increasing statistical power and offering a holistic view. Existing multi-variate association methods mostly focus on SNP-based genetic associations.

Genome-wide analyses identify novel risk loci for cluster headache in Han Chinese residing in Taiwan.

Background: Cluster headache is a highly debilitating neurological disorder with considerable inter-ethnic differences. Genome-wide association studies (GWAS) recently identified replicable genomic loci for cluster headache in Europeans, but the genetic underpinnings for cluster headache in Asians remain unclear. The objective of this study is to investigate the genetic architecture and susceptibility loci of cluster headache in Han Chinese resided in Taiwan.

Efficiently Predicting Vancomycin Resistance of From MALDI-TOF MS Spectra Using a Deep Learning-Based Approach.

Matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) has recently become a useful analytical approach for microbial identification. The presence and absence of specific peaks on MS spectra are commonly used to identify the bacterial species and predict antibiotic-resistant strains. However, the conventional approach using few single peaks would result in insufficient prediction power without using complete information of whole MS spectra.

Network hub-node prioritization of gene regulation with intra-network association.

Background: To identify and prioritize the influential hub genes in a gene-set or biological pathway, most analyses rely on calculation of marginal effects or tests of statistical significance. These procedures may be inappropriate since hub nodes are common connection points and therefore may interact with other nodes more often than non-hub nodes do. Such dependence among gene nodes can be conjectured based on the topology of the pathway network or the correlation between them.

Clinical practice guidelines in severe traumatic brain injury in Taiwan.

Background: Severe TBIs are major causes of disability and death in accidents. The Brain Trauma Foundation supported the first edition of the Guidelines for the Management of Severe Traumatic Brain Injury in 1995 and revised it in 2000. The recommendations in these guidelines are well accepted in the world.

Survey of traumatic intracranial hemorrhage in Taiwan.

Background: Injury ranked third among the top 10 leading causes of death in Taiwan from 1964 to 1996 and is still among the top 10 presently. Among transportation accidents, motor vehicle-related injury had the highest incidence rate, often resulting in traumatic head injury.

Methods: This survey was conducted from July 1, 1994, to June 30, 2002, and was collected from 55 major hospitals in Taiwan.

Head injuries in adolescents in Taiwan: a comparison between urban and rural groups.

Background: Data pertaining to head injuries in adolescents in Taiwan are scarce. The purpose of this study was to investigate the trend and pattern of head injuries in adolescents in both urban and rural areas in Taiwan.

Methods: We collected data from major hospitals in the urban (20) and in the rural (4) areas of Taiwan for a period of 3 years.

Pediatric traumatic brain injuries in Taiwan: an 8-year study.

Background. This study is intended to determine the causes of pediatric traumatic brain injuries (PTBI) in children aged 14 years or less, and to identify various types of craniocerebral damage resulting from different mechanisms of injury.Methods.