Publications by authors named "Huizhu Nan"

Article Synopsis
  • - Neospora caninum, a protozoan parasite, relies on complex signaling for its life processes, but research on the transcriptional regulation by the AP2 family, particularly NcAP2XII-4, has been limited.
  • - The study aimed to characterize the NcAP2XII-4 gene, including its expression and role in transcriptional regulation, using techniques like CRISPR/Cas9 to create a mutant strain and various assays for protein analysis.
  • - Findings indicated that NcAP2XII-4 encodes a large protein with nuclear localization, and its deletion caused significant growth issues in the parasite, demonstrating its essential role in N. caninum's development.
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Blastocystis sp. is a protozoan parasite that infects the intestines of humans and animals, causing chronic diseases such as skin rashes, abdominal pain, and irritable bowel syndrome. A survey was conducted to determine the prevalence and genetic diversity of Blastocystis sp.

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A real-time PCR assay was developed for specific detection of novel duck-origin goose parvovirus (N-GPV), the etiological agent of duck beak atrophy and dwarfism syndrome (BADS). The detection limit of the assay was 10 copies. The assay was useful in the prevention and control of BADS.

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Neospora caninum, an Apicomplexa parasite, is the causative agent of neosporosis. As described for other members of Apicomplexa, microneme proteins (MICs) play a key role in attachment and invasion of host cells by N. caninum.

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High mobility group box 1 (HMGB1) is a nuclear factor that usually binds DNA and modulates gene expression in multicellular organisms. Three HMGB1 orthologs were predicted in the genome of Toxoplasma gondii, an obligate intracellular protozoan pathogen, termed TgHMGB1a, b and c. Phylogenetic and bioinformatic analyses indicated that these proteins all contain a single HMG box and which shared in three genotypes.

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Toxoplasma gondii (T. gondii) and Neospora caninum (N. caninum) are both obligate intracellular protozoan parasites and share many common morphological and biological features.

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