Fabrication of bifunctional G-quadruplex-hemin DNAzymes for colorimetric detection of apurinic/apyrimidinic endonuclease 1 and microRNA-21.

G-quadruplex-based complexes have been widely used in various analytical methods due to their outstanding capabilities of generating colorimetric, fluorescent or electrochemical signals. However, since loop sequences in traditional G-quadruplex structures are quite short, it is difficult to establish biosensors solely using G-quadruplex-based complexes. Herein, we attempted to lengthen the loop sequences of G-quadruplex structures and found that G-quadruplex-hemin DNAzymes (G-DNAzymes) with long loops (even 30 nucleotides) maintain high peroxidase activity.

Palladium-catalyzed direct and regioselective C-H acyloxylation of indolizines.

A direct and regioselective C1-acyloxylation of indolizines was developed via palladium-catalyzed C-H functionalization. A series of indolizines were successfully acyloxylated at the C1 position with the tolerance of a broad range of functional groups. In this reaction, high regioselectivity was achieved in the absence of a directing group.

A new PEG-1000-based dicationic ionic liquid exhibiting temperature-dependent phase behavior with toluene and its application in one-pot synthesis of benzopyrans.

A new recyclable temperature-dependant phase-separation system comprised of PEG-1000-based dicationic acidic ionic liquid and toluene was developed and used in one-pot three-component condensation to prepare benzopyrans in excellent yields (up to 93%).