DF-1-Derived exosomes mediate transmission of reticuloendotheliosis virus and resist REV-specific antibodies.

Background: Reticuloendotheliosis virus (REV), a member of the family Retroviridae, is a hot area of research, and a previous study showed that exosomes purified from REV-positive semen were not blocked by REV-specific neutralizing antibodies and established productive infections.

Methods: To further verify the infectivity of exosomes from REV-infected cells, we isolated and purified exosomes from REV-infected DF-1 cells and identified them using Western blot and a transmission electron microscope. We then inoculated 7-day-old embryonated eggs, 1-day-old chicks and 23-week-old hens with and without antibody treatment.

The relationship between nurses' social network degree centrality and organizational citizenship behavior: The multiple mediating effects of job satisfaction and work engagement.

Background: In nursing care organizations, nurses' social networks affect their behavior and play an important role in nursing practice. This study aimed to explore the relationships among social network degree centrality, job satisfaction, work engagement and organizational citizenship behavior (OCB) among nurses.

Methods: A cross-sectional survey design (one-on-one investigation) was used.

Rereferencing of clinical EEGs with nonunipolar mastoid reference to infinity reference by REST.

Objective: Conventional electroencephalography (EEG) offline subtraction rereferencing is invalid for many clinical practices when adopting a specific nonunipolar recording montage (e.g., the ipsilateral mastoid (IM) and contralateral mastoid (CM)).

Interactions of microplastics with organic, inorganic and bio-pollutants and the ecotoxicological effects on terrestrial and aquatic organisms.

As emerging contaminants, microplastics (MPs) have attracted global attention. They are a potential risk to organisms, ecosystems and human health. MPs are characterized by small particle sizes, weak photodegradability, and are good environmental carriers.

soil moisture and temperature network in genhe watershed and saihanba area in China.

The dataset presented in this article is related to the work "Evaluation and Analysis of SMAP, AMSR2, and MEaSUREs Freeze/Thaw Products in China [1]". Soil moisture and temperature are important variables of land-atmosphere energy exchange, monitoring vegetation growth, predicting drought disasters and climate and hydrological modelling [2], [3], [4], [5], [6]. This work provides detailed information on soil moisture and temperature data network established in the Genhe watershed and Saihanba area in China, respectively.

High-mobility group box 1 protein (HMGB1) from Cherry Valley duck mediates signaling pathways and antiviral activity.

High-mobility group box 1 protein (HMGB1) shows endogenous damage-associated molecular patterns (DAMPs) and is also an early warning protein that activates the body's innate immune system. Here, the full-length coding sequence of HMGB1 was cloned from the spleen of Cherry Valley duck and analyzed. We find that duck HMGB1(duHMGB1) is mostly located in the nucleus of duck embryo fibroblast (DEF) cells under normal conditions but released into the cytoplasm after lipopolysaccharide (LPS) stimulation.