The integration of psychology and medicine: an empirical study of curriculum reform from the perspective of China.

Under the background of new medicine, innovative reform of medical education is mushrooming in Mainland, China. New medicine advocates an innovative training mode supported by medicine + X discipline. In the present study, we made use of the advantages of medical colleges to reform the curriculum of applied psychology and constructed an innovative curriculum system by integrating medicine with psychology.

Insights into the role of electrochemical stimulation on sulfur-driven biodegradation of antibiotics in wastewater treatment.

The presence of antibiotics in wastewater poses significant threat to our ecosystems and health. Traditional biological wastewater treatment technologies have several limitations in treating antibiotic-contaminated wastewaters, such as low removal efficiency and poor process resilience. Here, a novel electrochemical-coupled sulfur-mediated biological system was developed for treating wastewater co-contaminated with several antibiotics (e.

Physiological responses and molecular mechanisms of biofilm formation induced by extracellular metabolites of euglena in Pseudomonas aeruginosa LNR1 for diesel biodegradation based on transcriptomic and proteomic.

Diesel, as a toxic and complex pollutant, is one of the main components in oily wastewater, and poses serious threats to the aquatic environment and the health of organisms. Employing environmentally friendly biostimulants to enhance the metabolic functions of microorganisms is currently the optimal choice to improve the biodegradation of oil-containing wastewater efficiency. This study takes Pseudomonas aeruginosa LNR1 as the target, analyzing the physiological responses and molecular mechanisms of biofilm formation when enhanced by the extracellular metabolites of euglena (EME) for diesel degradation.

Comprehensive assessment of toxicity and environmental risk associated with sulfamethoxazole biodegradation in sulfur-mediated biological wastewater treatment.

Incomplete mineralization of sulfamethoxazole (SMX) in wastewater treatment systems poses a threat to ecological health. The toxicity and environmental risk associated with SMX biodegradation in the sulfur-mediated biological process were examined for the first time through a long-term (180 days) bioreactor study and a series of bioassays. The results indicated that the sulfur-mediated biological system was highly resistant and tolerant to SMX toxicity, as evidenced by the enrichment of sulfate-reducing bacteria (SRB), the improved microbial metabolic activity, and the excellent performance on pollutants removal under long-term SMX exposure.

Unraveling the Toxicity Associated with Ciprofloxacin Biodegradation in Biological Wastewater Treatment.

Incomplete mineralization of antibiotics in biological sludge systems poses a risk to the environment. In this study, the toxicity associated with ciprofloxacin (CIP) biodegradation in activated sludge (AS), anaerobic methanogenic sludge (AnMS), and sulfur-mediated sludge (SmS) systems was examined via long-term bioreactor tests and a series of bioassays. The AS and AnMS systems were susceptible to CIP and its biotransformation products (TPs) and exhibited performance deterioration, while the SmS system exhibited high tolerance against the toxicity of CIP and its TPs along with excellent pollutant removal.

Enhanced performance and electron transfer of sulfur-mediated biological process under polyethylene terephthalate microplastics exposure.

Microplastics are ubiquitous in estuaries, coasts, sewage and wastewater treatment plants (WWTPs), which could arouse unexpected effects on critical microbial processes in wastewater treatment. In this study, polyethylene terephthalate microplastics (PET-MPs) were selected to investigate the mechanism of its influence on the performance of sulfur-mediated biological process from the perspective of microbial metabolic activity, electron transfer capacity and microbial community. The results indicated that the exposure of 50 particles/L PET-MPs improved the chemical oxygen demand (COD) and sulfate removal efficiencies by 6.

Biotransformation of ibuprofen in biological sludge systems: Investigation of performance and mechanisms.

Ibuprofen (IBU), a common non-steroidal anti-inflammatory drug (NSAID), is widely used by humans for controlling fever and pain, and is frequently detected in the influent of wastewater treatment plants and different aquatic environments. In this study, the biotransformation of IBU in activated sludge (AS), anaerobic methanogenic sludge (AnMS) and sulfate-reducing bacteria (SRB)-enriched sludge systems was investigated at three different concentrations of 100, 500 and 1000 μg/L via a series of batch and continuous studies. IBU at concentration of 100 μg/L was effectively biodegraded by AS whereas AnMS and SRB-enriched sludge were less effective in IBU biodegradation at all concentrations tested.

Stress-responses of activated sludge and anaerobic sulfate-reducing bacteria sludge under long-term ciprofloxacin exposure.

The activated sludge (AS) and sulfate-reducing bacteria (SRB) sludge systems were continuously operated for 200 days in laboratory to investigate the stress-responses of these two sludge systems under ciprofloxacin (CIP) exposure. It was found that CIP was effectively removed by SRB sludge via adsorption and biodegradation, but little biodegradation in AS system. The CIP biodegradation by SRB sludge made the SRB sludge system more sustainable and tolerant to long-term CIP exposure than AS system with significant (p < 0.

Insights into the Fate and Removal of Antibiotics in Engineered Biological Treatment Systems: A Critical Review.

Antibiotics, the most frequently prescribed drugs of modern medicine, are extensively used for both human and veterinary applications. Antibiotics from different wastewater sources (e.g.

Insights into pharmaceuticals removal in an anaerobic sulfate-reducing bacteria sludge system.

In this study, we examined eight typical and widely detected pharmaceuticals (PhAs) removal in an anaerobic sulfate-reducing bacteria (SRB) sludge system (five antibiotics: sulfadiazine (SD), sulfamethoxazole (SMX), trimethoprim (TMP), ciprofloxacin (CIP) and enoxacin (ENO), and three nonsteroidal anti-inflammatory drugs (NSAIDs): ibuprofen (IBU), ketoprofen (KET) and diclofenac (DIC)). The results showed that the SRB sludge had the higher removal efficacy (20 to 90%) for antibiotics (SD, SMX, TMP, CIP and ENO) than NSAIDs (<20%) via adsorption and biodegradation under different operating conditions. Based on a series of batch studies, fluoroquinolone antibiotics (CIP and ENO) were instantly (<15 min) removed (∼98%) via adsorption on SRB sludge with adsorption coefficient (K) as high as 25.

A Unique ATPase, ArtR (PA4595), Represses the Type III Secretion System in .

is an important human pathogen which uses the type III secretion system (T3SS) as a primary virulence factor to establish infections in humans. The results presented in this report revealed that the ATP-binding protein PA4595 (named ArtR, a Regulator that is an ATP-activated Repressor of T3SS) represses T3SS expression in . The expression of T3SS genes, including , , , and -, increased significantly when was knockout.

Regulatory Effect of DNA Topoisomerase I on T3SS Activity, Antibiotic Susceptibility and Quorum- Sensing-Independent Pyocyanin Synthesis in .

Topoisomerases are required for alleviating supercoiling of DNA during transcription and replication. Recent evidence suggests that supercoiling of bacterial DNA can affect bacterial pathogenicity. To understand the potential regulatory role of a topoisomerase I (TopA) in we investigated a previously isolated mutation using genetic approaches.

[Adsorption Mechanisms of Ciprofloxacin by Extracellular Polymeric Substances of Sulfate-reducing Bacteria Sludge].

Extracellular polymeric substances (EPS) in microbial sludge, fulfils a key role in removal of micro-organic pollutants during biological wastewater treatment. In this study, the authors evaluated the removal of ciprofloxacin (CIP) by sulfate-reducing bacteria (SRB) sludge in a sulfate-reducing up-flow sludge bed (SRUSB) reactor, and examined the role of EPS on CIP removal in an SRB sludge system. The results indicated that CIP was removed efficiently through adsorption and biodegradation by SRB sludge, with adsorption the major removal pathway.

Understanding the Role of Extracellular Polymeric Substances on Ciprofloxacin Adsorption in Aerobic Sludge, Anaerobic Sludge, and Sulfate-Reducing Bacteria Sludge Systems.

Extracellular polymeric substances (EPS) of microbial sludge play a crucial role in removal of organic micropollutants during biological wastewater treatment. In this study, we examined ciprofloxacin (CIP) removal in three parallel bench-scale reactors using aerobic sludge (AS), anaerobic sludge (AnS), and sulfate-reducing bacteria (SRB) sludge. The results showed that the SRB sludge had the highest specific CIP removal rate via adsorption and biodegradation.

Sulfamethoxazole degradation in anaerobic sulfate-reducing bacteria sludge system.

Sulfamethoxazole (SMX) is one of the most commonly used antibiotics. SMX degradation in sulfate-reducing bacteria (SRB) sludge systems has not been reported so far. This research investigated the SMX degradation using SRB sludge in a sulfate-reducing up-flow sludge bed reactor.

Rapid screening of toxic salbutamol, ractopamine, and clenbuterol in pork sample by high-performance liquid chromatography-UV method.

A rapid and simple high-performance liquid chromatography-UV method was developed for the separation and quantification of salbutamol, ractopamine, and clenbuterol in pork. A mixture of acetonitrile-formic acid-ammonium acetate was used as the mobile phase to separate three β-agonists on a C18 column with gradient. The effects of the addition of formic acid and ammonium acetate to mobile phases on the separation of β-agonists were investigated.