Dynamic changes in plasma tissue plasminogen activator, plasminogen activator inhibitor-1 and beta-thromboglobulin content in ischemic stroke.

The aim of this paper is to investigate the corresponding variations of plasma tissue plasminogen activator (t-PA) and plasminogen activator inhibitor-1 (PAI-1) activities, and beta-thromboglobulin (β-TG) content in patients during different stages of ischemic stroke. Ischemic stroke is a common disease among aging people and its occurrence is associated with abnormalities in the fibrinolytic system and platelet function. However, few reports focus on the dynamic changes in the plasma fibrinolytic system and β-TG content in patients with ischemic stroke.

Overexpression of arginine transporter CAT-1 is associated with accumulation of L-arginine and cell growth in human colorectal cancer tissue.

We previously showed that L-arginine (Arg) accumulates in colorectal cancer tissues. The aim of this study was to investigate the mechanism by which Arg accumulates and determine its biological significance. The concentration of Arg and Citrulline (Cit) in sera and tumor tissues from colorectal cancer (CRC) patients was analyzed by high-performance liquid chromatography (HPLC).

A novel spectrophotometric method for indirect determination of nitric oxide (NO) in serum.

Background: It is essential in clinical medicine to develop a simple and accurate analytical method for detecting nitric oxide (NO) in blood or other body fluid. In order to overcome the shortcomings of previous methods, this experiment developed a novel indirect analytical method of NO in serum.

Methods: After nitrate was reduced by copper-coated cadmium granules, the nitrite produced was determined by diazotization of fuchsin acid and coupling to resorcinol.

Syk protein tyrosine kinase involves PECAM-1 signaling through tandem immunotyrosine inhibitory motifs in human THP-1 macrophages.

Although recent evidence supports a functional relationship between platelet endothelial cell adhesion molecule (PECAM-1) and Syk tyrosine kinase, little is known about the interaction of Syk with PECAM-1. We report that down-regulation of Syk inhibits the spreading of human THP-1 macrophage cells. Moreover, our data indicate that Syk binds PECAM-1 through its immune tyrosine-based inhibitory motif (ITIM), and dual phosphorylation of the ITIM domain of PECAM-1 leads to activation of Syk.

Antibody-mediated platelet phagocytosis by human macrophages is inhibited by siRNA specific for sequences in the SH2 tyrosine kinase, Syk.

Immune thrombocytopenia depends upon Fc receptor-mediated phagocytosis that involves signaling through the SH2 tyrosine kinase, Syk. We designed small interfering (siRNA) sequences complementary to Syk coding regions to decrease the expression of Syk in the human macrophage cell line, THP-1. To evaluate the functional effect of siRNA on phagocytosis, we developed a new in vitro assay for antibody-mediated platelet ingestion by THP-1 cells.

Simultaneous determination of l-citrulline and l-arginine in plasma by high performance liquid chromatography.

Objective: To develop a method for simultaneously determining l-citrulline and L-arginine levels in plasma using RP-HPLC with ultraviolet detection.

Design And Methods: Plasma samples were deproteinized by trichloroacetic acid and heat. Phenyl-isothiocyanate (PITC) solution was used as derivatization reagent and a gradient elution was carried out.

Macroscopic samples of polystyrene with ordered three-dimensional nanochannels.

Block copolymers containing a chemically degradable block are versatile precursors to nanoporous organic materials. Most work in this area has been accomplished in thin films. However, high surface area catalysis, separations, and nanotemplating can require monolithic samples with macroscopic pore volumes.

Control of pore hydrophilicity in ordered nanoporous polystyrene using an AB/AC block copolymer blending strategy.

Ordered nanoporous plastics with hydrophilic pore surfaces were prepared by the degradative removal of polylactide from a self-organised, multi-component composite containing two block copolymers: polystyrene-polylactide and polystyrene-polyethylene oxide. The solid-state characterization of blends containing up to 12 wt.% polyethylene oxide was consistent with nanoscopic cylinders of mixed polyethylene oxide and polylactide hexagonally packed in a polystyrene matrix.