Imparting insoluble-soluble property to Cyt c by immobilizing Cyt c in UCST-pH dual responsive polymer for highly sensitive detection of phenol.

Immobilization of enzymes in porous organic framework (POF) materials is popular strategy to stabilize enzymes. For such solid enzyme catalysis system, improving the catalytic efficiency is challenging due to the diffusion resistance from solid-liquid interface and inner pores. Here, UCST-pH dual responsive polymeric carrier (PEG-b-PAAm-b-P(GMA-co-AAc)) was synthesized to immobilize cytochrome c (Cyt c), which impart the reversibly insoluble-soluble property to the immobilized Cyt c.

Fabricating Polymeric Micelles with Enrichment and Cavity Effect for In Situ Enzyme Imobilization from Natural Biosystems.

Metal-organic frameworks and hydrogen-organic frameworks (MOFs and HOFs) are attractive hosts for enzyme immobilization, but they are limited to immobilizing the purified enzymes, making industrial upscaling unattractive. Herein, aptamer-modified dual thermoresponsive polymeric micelles with switchable self-assembly and core-shell structure are constructed, which enable selective immobilization of trypsin directly from complex biological systems through a cascade operation of separation and immobilization. Their steric self-assembly provides a large amount of adsorption sites on the soluble micellar shell, resulting in high adsorption capacity and excellent selectivity.

A new type II CHH neuropeptide involves ovarian development in the peppermint shrimp, Lysmata vittata.

Type II crustacean hyperglycemic hormone (CHH) neuropeptides play diverse roles in crustaceans. In the hermaphrodite shrimp Lysmata vittata, two transcripts of type II CHHs (molt-inhibiting hormone/gonad-inhibiting hormone, MIH/GIH1 and MIH/GIH2) were identified by transcriptome sequencing, and MIH/GIH1 was later named Lvit-GIH1 for its inhibitory effect on ovarian development. Based on the high similarity of MIH/GIH2 to Lvit-GIH1, we named tentatively MIH/GIH2 as Lvit-GIH2 and explored the role of Lvit-GIH2 in ovarian development.