The nonlinear correlation between the cardiometabolic index and the risk of diabetes: A retrospective Japanese cohort study.

Background: The cardiometabolic index (CMI) has been proposed as a novel indicator of cardiometabolic status. However, evidence on the relationship between CMI and diabetes mellitus (DM) risk was limited. Our study aimed to explore the relationship between CMI and DM risk among a large cohort of Japanese adults.

[Value of pre-gestational deafness-related mutation screening for the prevention and intervention of congenital deafness].

Objective: To assess the value of pre-gestational deafness-related mutation screening for the prevention and intervention of congenital deafness.

Methods: In this study, 2168 couples with normal hearing were screened for common mutations associated with congenital deafness using real-time fluorescence quantitative PCR. The mutations have included GJB2 c.

[Construction of cell surface display system in lactic acid bacteria by using ice nucleation protein].

Objective: The gene of ice nucleation protein (INP), an outer membrane of Pseudomonas syringae was tested to display foreign proteins on the surface of Lactococcus lactis.

Methods: Plasmids pHZ101 and pHZ102 were constructed using gfp (Green Fluorescence Protein) as the reporter gene and N-terminal and NC-terminal of inp as the anchoring units. Plasmids pHZ101 and pHZ102 were subsequently transformed into Escherichia coli JM109 and Lactococcus lactis MG1363.