Publications by authors named "Huike Ye"

The significant influx of antibiotics into the environment represents ecological risks and threatens human health. Microbial degradation stands as a highly effective method for reducing antibiotic pollution. This study explored the potential of immobilized microbial consortia to efficiently degrade tetracycline.

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Anthropogenic activities are driving significant changes in coastal ecological environments, increasingly spotlighting microorganisms associated with seagrass bed ecosystems. is primarily recognized as a saprophytic protist associated with marine detritus, and it also acts as an opportunistic pathogen affecting marine algae, terrestrial plants and mollusks, especially in coastal environments. The genus plays a key role in the decomposition of marine detritus, facilitated by its interactions with diatoms and through the utilization of a diverse array of carbohydrate-active enzymes to decompose seagrass cell walls.

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With the widespread use of tetracycline antibiotics (TCs) and the application of manure fertilizer in farmland, TCs and their metabolites especially 4-epimers have been heavily detected in agricultural soil. However, existing studies have focused on the residual and environmental behavior of maternal TCs, and few studies have looked at the ecotoxicity of their 4-epimers in soil. In this study, the degradation and interconversion of tetracycline (TC), oxytetracycline (OTC) and their 4-epimers (4-epitetracycline, ETC; 4-epioxytetracycline, OTC) were revealed.

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Article Synopsis
  • Organic fertilization significantly boosts the abundance of soil antibiotic resistance genes (ARGs) compared to no fertilization or commercial fertilizers, yet it shows a weaker transfer of these resistance genes to crops.
  • The study reveals that organic fertilizers enrich the soil with nutrients, creating conditions (neutral pH, low salt, and mesotrophic carbon content) that enhance the persistence of resistant plasmids in the soil microbiome but limit their spread to plant endophytes.
  • Understanding how organic fertilization impacts antibiotic resistance can help develop better strategies to manage antimicrobial resistance in agriculture.
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Background: Saline lakes are home to various archaea that play special and crucial roles in the global biogeochemical cycle. The Qinghai-Tibet Plateau hosts a large number of lakes with diverse salinity ranging from 0.1 to over 400 g/L, harboring complex and diverse archaea.

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Microplastics (MPs) pose a threat to farmland soil quality and crop safety. MPs exist widely in food legumes farmland soil due to the extensive use of agricultural film and organic fertilizer, but their distribution characteristics and their impact on soil environment have not been reported. The abundance and characteristics of MPs, soil physical and chemical properties, and bacterial community composition were investigated in 76 soil samples from five provinces in northern China.

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Humus substances (HSs) participate in extracellular electron transfer (EET), which is unclear in heterogeneous soil. Here, a microbial electrochemical system (MES) was constructed to determine the effect of HSs, including humic acid, humin and fulvic acid, on soil electron transfer. The results showed that fulvic acid led to the optimal electron transfer efficiency in soil, as evidenced by the highest accumulated charges and removal of total petroleum hydrocarbons after 140 days, with increases of 161% and 30%, respectively, compared with those of the control.

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Bioelectric field is a stimulated force to degrade xenobiotic pollutants in soils. However, the effect of bioelectric field on microplastics (MPs) aging is unclear. The degradation behavior of polyvinyl chloride (PVC), polyethylene (PE) and polylactic acid (PLA) was investigated in an agricultural soil microbial electrochemical system in which bioelectric field was generated in-situ by native microbes.

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Article Synopsis
  • Thraustochytrids, specifically the Aurantiochytrium PKU#Mn16 strain, produce high levels of docosahexaenoic acid (DHA), and their growth is influenced by dissolved oxygen (DO) levels during fermentation.
  • Experimenting with three different DO conditions (10%, 30%, and 50%), researchers found that 10% DO improved long-term biomass and DHA production despite slower initial growth.
  • The study also discovered that genes related to metabolism were downregulated at low DO, indicating reduced oxidative damage, and highlights the potential for optimizing DHA production through bioprocess engineering by maintaining low oxygen levels.
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To explore the responses of soil microbial communities to concentration gradients of antibiotic residues in soil, 32 soil samples were collected from a typical greenhouse vegetable production base in Northern China in 2019. The total concentrations of 26 antibiotic residues in these soil samples was 83.24-4237.

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The redox process driven by anaerobic respiration is a link between matter conversion and energy exchange in soil biogeochemistry. Microbial extracellular electron transfer forming biocurrents is a force in element cycling and community living in soil. However, the effect of indigenous microorganisms generating biocurrents on soil quality is unclear.

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Thraustochytrids have gained significant attention in recent years because of their considerable ecological and biotechnological importance. Yet, the influence of seasons and habitats on their culturable diversity and lipid profile remains poorly described. In this study, a total of 58 thraustochytrid strains were isolated from the coastal waters of Qingdao, China.

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Article Synopsis
  • The study explores how different carbon and nitrogen sources affect the production of DHA (docosahexaenoic acid) in a particular strain of thraustochytrids, highlighting the role of PUFA synthase.
  • Significant variations were found in the expression of the pfaB gene, which is involved in DHA production, depending on the carbon and nitrogen sources used, but these changes did not always align with DHA output.
  • Nitrogen starvation was shown to boost DHA production, indicating the importance of PUFA synthase activity under nutrient-limited conditions, and the study confirms the pfaB gene’s role in this process.
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This study reports comparative evaluation of the growth and DHA productivity of the thraustochytrid strain Thraustochytriidae PKU#Mn16 fermented with seven different substrate feeding strategies. Of these strategies, fed-batch fermentation of the mixed substrate (glucose & glycerol) yielded the maximum growth (52.2 ± 1.

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Labyrinthulomycete protists have gained significant attention in the recent past for their biotechnological importance. Yet, their lipid profiles are poorly described because only a few large-scale isolation attempts have been made so far. Here, we isolated more than 200 strains from mangrove habitats of China and characterized the molecular phylogeny and lipid accumulation potential of 71 strains.

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Thraustochytrids, rich in docosahexaenoic acid (DHA, C22:6ω3), represent a potential source of dietary fatty acids. Yet, the effect of culture conditions on growth and fatty acid composition vary widely among different thraustochytrid strains. Two different thraustochytrid strains, sp.

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Core endpoints in zebrafish embryos are crucial indicators in screening harmful effects of chemicals. In this study, we established a three-step process to more quantitatively and less-subjective determine effects of chemicals on phenotypes of developing zebrafish embryos. Embryos were exposed to each of two concentrations of the representative chemicals cadmium chloride (CdCl), tris(1,3-dichloro-2-propyl) phosphate (TDCIPP) or 1H, 1H, 2H, 2H-nonafluoro-1-hexanol (4:2 FTOH) from 0.

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