Publications by authors named "Huijun Zong"
Background: Human neutrophil lipocalin (HNL) is a marker of neutrophil activation and has a high efficacy in diagnosing bacterial infections. In this study, we applied the AlphaLISA technique to measure the serum level of HNL, evaluate HNL's efficacy in diagnosing septic shock, and identify any association between HNL level and septic patients' prognosis.
Methods: We collected 146 serum samples from the Fifth Medical Center of Chinese PLA General Hospital.
Objective: Influenza B virus (IBV) is highly contagious, spreads rapidly, and causes seasonal epidemic respiratory disease in the human population, especially in immunocompromised people and young children. Clinical manifestations in this high-risk population are often more severe than in immunocompetent hosts and sometimes atypical. Therefore, rapid, and accurate detection of IBV is important.
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- This study focuses on the need for quick and effective diagnosis of pathogens and antibiotic resistance in patients with ventilator-associated pneumonia (VAP) using a new nanopore-based metagenomic next-generation sequencing (mNGS) method.
- Data was collected from 63 patients, and the mNGS test achieved significant sensitivity (91.3%) and specificity (78.3%) when compared to traditional methods, also demonstrating faster results with a turnaround time of about 4.43 hours.
- The mNGS method showed promise in identifying viral infections and antimicrobial resistance genes, highlighting higher resistance in late-onset VAP cases while demonstrating an increased detection rate of coinfections.
Rotavirus is the main pathogen causing acute viral gastroenteritis. Accurate and rapid diagnosis of rotavirus infection is important to determine appropriate treatment, prevention of unnecessary antibiotics use and control of infection spread. In this study, we established a rapid, accurate, and sensitive amplified luminescent proximity homogeneous assay linked immunosorbent assay (AlphaLISA) for detecting rotavirus and evaluated its efficacy in human stool samples.
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