[Preparation and physicochemical properties for zirconia as high performance liquid chromatographic packing materials].

There has been an increasing importance in the application of zirconia as column packing material for high performance liquid chromatography (HPLC) because of its excellent chemical, thermal, and mechanical stabilities as well as bioanalysis suitability. Zirconia spheres could be synthesized by polymerization-induced colloid aggregation (PICA), oil emulsion methods (OEM), or spray-dry method. It has been found that preparative procedure of zirconia support can affect its chromatographic behavior significantly.

[Preparation of polymer modified stationary phases through surface radical chain transfer reaction].

It is demonstrated that radical chain transfer to thiol-terminated self-assembled monolayer and surface-initiated polymerization could be used for in-situ grafting ultra-thin polymer film on silica. The widely used silane coupling agent mercaptopropyltrimethoxysilane (MPS) was used to prepare the thiol-terminated silica, and in the presence of which, chain-transfer reaction and polymerization of methyl methacrylate (MMA) was carried out using azo di-isobutylnitrile (AIBN) as the initiator. Both thiol-terminated silica and polymer film modified silica were characterized by Fourier transform infrared spectroscopy (FTIR), Raman spectroscopy, thermogravimetric analysis (TGA) and elemental analysis.

[Resonance light scattering of 4-(2-pyridylazo)-resorcinol (PAR)].

Mechanism and influence factors of resonance light scattering (RLS) spectra of 4-(2-pyridylazo)-resorcinol (PAR) aqueous solution were studied. In weak acidic solutions, strong RLS of PAR can be observed. The spectral feature of RLS is related to the absorption spectra of PAR.

[Influence of mobile phase composition on chiral separation of albendazole sulfoxide racemates].

A chiral stationary phase (CSP) was prepared by coating amylose-tris(3,5-dimethylphenyl carbamate) (ADMPC) onto aminopropylated spherical silica gel. Albendazole sulfoxide (ABZSO) was directly resolved on the ADMPC-CSP by normal-phase high performance liquid chromatography (HPLC) using a series of binary and ternary mobile phases. The results indicated that the retention and stereoselectivity of ABZSO on ADMPC-CSP were significantly influenced by the kinds and the contents of the alcohols in mobile phases.

Layer-by-layer self-assembly of multilayer zirconia nanoparticles on silica spheres for HPLC packings.

A novel zirconia-based HPLC packing material, ZrO2/SiO2, which consists of micrometer-sized silica spheres as core and nanometer-sized zirconia particles as surface coating, was prepared by a layer-by-layer self-assembly technique. The material exhibits favorable characteristics for HPLC applications, including high surface area and pore volume, good pore structure, narrow particle size, and pore size distribution. Not only the support ZrO2/SiO2 but also the stationary-phase C18 bonded ZrO2/SiO2 exhibits excellent chemical stability.

[Influence of mobile phase composition on chiral separation of organic selenium racemates].

The chiral separation of some chiral compounds with similar structure on the cellulose tris (3,5-dimethylphenylcarbamate) chiral stationary phase prepared by us was obtained. Ternary mobile phases influencing chiral recognition were investigated. A mode of interaction between the structural character of samples and chiral stationary phase is discussed.