Dynamic visualization of endoplasmic reticulum viscosity in living cells with a multirotor-based fluorescent probe.

Changes in viscosity can significantly influence the functionality of the endoplasmic reticulum (ER), and viscosity-responsive fluorescent probes can provide valuable feedback on its physiological state. In this study, three multirotor-based fluorescent probes (1-3) were designed and synthesized. Probes 1 and 2 exhibited remarkable viscosity sensitivity and specific targeting ability towards the ER.

pXST, a novel vector for TA cloning and blunt-end cloning.

Background: With the rapid development of sequencing technologies, increasing amount of genomic information has been accumulated. To clone genes for further functional studies in large scale, a cheap, fast and efficient cloning vector is desired.

Results: A bifunctional vector pXST has been constructed.

Genome-Wide Identification, Expression, and Functional Analysis of the Sugar Transporter Gene Family in Cassava (Manihot esculenta).

The sugar transporter () gene family encodes monosaccharide transporters that contain 12 transmembrane domains and belong to the major facilitator superfamily. genes play critical roles in monosaccharide distribution and participate in diverse plant metabolic processes. To investigate the potential roles of in cassava () tuber root growth, genome-wide identification and expression and functional analyses of the gene family were performed in this study.