Testing the carbohydrate-insulin model: Short-term metabolic responses to consumption of meals with varying glycemic index in healthy adults.

The carbohydrate-insulin model predicts that meals with varying glycemic indices will elicit distinct metabolic and hunger responses, including greater intake at subsequent meals following high-glycemic-index meals. To test this, a randomized trial (NCT05804942) was conducted in healthy adults using intervention meals with low, medium, and high glycemic indices and a constant macronutrient composition. After intake of the intervention meals, glucose and insulin followed the predicted pattern, but subjective hunger did not.

Deep Learning-Assisted Automated Multidimensional Single Particle Tracking in Living Cells.

The translational and rotational dynamics of anisotropic optical nanoprobes revealed in single particle tracking (SPT) experiments offer molecular-level information about cellular activities. Here, we report an automated high-speed multidimensional SPT system integrated with a deep learning algorithm for tracking the 3D orientation of anisotropic gold nanoparticle probes in living cells with high localization precision (<10 nm) and temporal resolution (0.9 ms), overcoming the limitations of rotational tracking under low signal-to-noise ratio (S/N) conditions.

A novel reversible fluorescent probe for Cu and Sions and imaging in living cells.

A novel fluorescent probe TSOC (thiazole salicylaldehyde oxazole chlorinated) was synthesized based on benzothiazole conjugated olefinic bonds with salicylicaldehyde unit as fluorophore and a phenyl oxazole unit as bonding unit. The probe could reversibly detect of Cuand Sover other common ions with longer emission and large stokes shift in an aqueous solution at pH 7.3 (DMSO-Hepes, v/v, 5:1, 10 mM).

A novel fluorescence probe for the selective detection of cysteine in aqueous solutions and imaging in living cells and mice.

A novel fluorescent probe based on hydroxyquinoline conjugated with a charged trimethylindolenine unit Toc-Ac was developed, which exhibited long wavelength emission (560 nm) and a large Stokes shift (∼140 nm) due to the intrinsic mechanism of the intramolecular charge transfer process. The probe Toc-Ac showed a highly sensitive response to Cys (the detection limit was 3.86 × 10 M) in aqueous solution and was successfully applied for detecting endogenous Cys in living cells and mice.

A hemicyanine-based "turn-on" fluorescent probe for the selective detection of Cu ions and imaging in living cells.

A novel hemicyanine-based fluorescent probe was developed for the fast, selective, and reversible detection of Cu ions with "turn-on" fluorescence response in the aqueous solution. The sensing mode was demonstrated through fluorescence, UV-vis, HRMS, H NMR, and DFT calculations. The probe was successfully used for the imaging of Cu ions in living cells.