CHI-KAT8i5 suppresses ESCC tumor growth by inhibiting KAT8-mediated c-Myc stability.

The integrated analysis of histone modifier enzymes in solid tumors, especially in esophageal squamous cell carcinoma (ESCC), is still inadequate. Here, we investigate the expression levels of histone modifier enzymes in ESCC tissues. Notably, KAT8 (lysine acetyltransferase 8) is identified as a prognostic and therapeutic biomarker in ESCC.

RTN4IP1 Contributes to ESCC via Regulation of Amino Acid Transporters.

Esophageal squamous cell carcinoma (ESCC) accounts for about 90% of esophageal cancer cases. The lack of effective therapeutic targets makes it difficult to improve the overall survival of patients with ESCC. Reticulon 4 Interacting Protein 1 (RTN4IP1) is a novel mitochondrial oxidoreductase.

Ribonucleotide reductase small subunit M2 promotes the proliferation of esophageal squamous cell carcinoma cells HuR-mediated mRNA stabilization.

Esophageal squamous cell carcinoma (ESCC), a malignancy of the digestive system, is highly prevalent and the primary cause of cancer-related deaths worldwide due to the lack of early diagnostic biomarkers and effective therapeutic targets. Dysregulated ribonucleotide reductase (RNR) expression has been confirmed to be causally linked to tumorigenesis. This study demonstrated that ribonucleotide reductase small subunit M2 (RRM2) is significantly upregulated in ESCC tissue and that its expression is negatively correlated with clinical outcomes.

Multi-omics characterization of esophageal squamous cell carcinoma identifies molecular subtypes and therapeutic targets.

Esophageal squamous cell carcinoma (ESCC) is the predominant form of esophageal cancer and is characterized by an unfavorable prognosis. To elucidate the distinct molecular alterations in ESCC and investigate therapeutic targets, we performed a comprehensive analysis of transcriptomics, proteomics, and phosphoproteomics data derived from 60 paired treatment-naive ESCC and adjacent nontumor tissue samples. Additionally, we conducted a correlation analysis to describe the regulatory relationship between transcriptomic and proteomic processes, revealing alterations in key metabolic pathways.

Erianin suppresses constitutive activation of MAPK signaling pathway by inhibition of CRAF and MEK1/2.

Constitutive activation of RAS-RAF-MEK-ERK signaling pathway (MAPK pathway) frequently occurs in many cancers harboring RAS or RAF oncogenic mutations. Because of the paradoxical activation induced by a single use of BRAF or MEK inhibitors, dual-target RAF and MEK treatment is thought to be a promising strategy. In this work, we evaluated erianin is a novel inhibitor of CRAF and MEK1/2 kinases, thus suppressing constitutive activation of the MAPK signaling pathway induced by BRAF V600E or RAS mutations.

Periplogenin suppresses the growth of esophageal squamous cell carcinoma in vitro and in vivo by targeting STAT3.

The mortality rate of esophageal squamous cell carcinoma (ESCC) is higher than that of other cancers worldwide owing to a lack of therapeutic targets and related drugs. This study aimed to find new drugs by targeting an efficacious therapeutic target in ESCC patients. Signal transducer and activator of transcription 3 (STAT3) is hyperactive in ESCC.

miR-17-3p promotes the proliferation of multiple myeloma cells by downregulating P21 expression through LMLN inhibition.

Multiple myeloma (MM), a hematological malignancy, has a poor prognosis and requires an invasive procedure. Reports have implicated miRNAs in the diagnosis, treatment and prognosis of hematological malignancies. In our study, we evaluated the expression profiles of miR-17-3p in plasma and bone marrow mononuclear cells of monoclonal gammopathy of undetermined significance (MGUS) and MM patients and healthy subjects.

Mitophagy receptor FUNDC1 is regulated by PGC-1α/NRF1 to fine tune mitochondrial homeostasis.

Mitophagy is an essential cellular autophagic process that selectively removes superfluous and damaged mitochondria, and it is coordinated with mitochondrial biogenesis to fine tune the quantity and quality of mitochondria. Coordination between these two opposing processes to maintain the functional mitochondrial network is of paramount importance for normal cellular and organismal metabolism. However, the underlying mechanism is not completely understood.

Catalytic route electrochemiluminescence microscopy of cell membranes with nitrogen-doped carbon dots as nano-coreactants.

Catalytic route electrochemiluminescence (ECL) microscopy enables imaging upper cell membranes with freely diffusing Ru(bpy) as the emitter and nitrogen-doped carbon dots as the nano-coreactants and labels. This strategy provides a vertical resolution when studying the ECL profiles at different heights and realizes the ECL imaging of the externalized phosphatidylserine.

Bio-Coreactant-Enhanced Electrochemiluminescence Microscopy of Intracellular Structure and Transport.

A bio-coreactant-enhanced electrochemiluminescence (ECL) microscopy realizes the ECL imaging of intracellular structure and dynamic transport. This microscopy uses Ru(bpy) as the electrochemical molecular antenna connecting extracellular and intracellular environments, and uses intracellular biomolecules as the coreactants of ECL reactions via a "catalytic route". Accordingly, intracellular structures are identified without using multiple labels, and autophagy involving DNA oxidative damage is detected using nuclear ECL signals.

Defective mitochondrial ISCs biogenesis switches on IRP1 to fine tune selective mitophagy.

Both iron metabolism and mitophagy, a selective mitochondrial degradation process via autolysosomal pathway, are fundamental for the cellular well-being. Mitochondria are the major site for iron metabolism, especially the biogenesis of iron-sulfur clusters (ISCs) via the mitochondria-localized ISCs assembly machinery. Here we report that mitochondrial ISCs biogenesis is coupled with receptor-mediated mitophagy in mammalian cells.

Hydrogen Evolution Reaction Monitored by Electrochemiluminescence Blinking at Single-Nanoparticle Level.

Monitoring and characterization methods that provide performance tracking of hydrogen evolution reaction (HER) at the single-nanoparticle level can greatly advance our understanding of catalysts' structure and activity relationships. Electrochemiluminescence (ECL) microscopy is implemented for the first time to identify HER activities of single nanocatalysts and to provide a direction for further optimization. Here, we develop a novel ECL blinking technique at the single-nanoparticle level to directly monitor H nanobubbles generated from hollow carbon nitride nanospheres (HCNSs).

Stable and Monochromatic All-Inorganic Halide Perovskite Assisted by Hollow Carbon Nitride Nanosphere for Ratiometric Electrochemiluminescence Bioanalysis.

Lead halide perovskites have been promising electrochemiluminescence (ECL) candidates because of their excellent photophysical attributes, but their poor stability has severely restricted ECL applications. Herein, the in situ assembly of all-inorganic perovskite CsPbBr nanocrystals (CPB) into hollow graphitic carbon nitride nanospheres (HCNS) were described as a novel ECL emitter. The architecture guaranteed not only improved stability because of the peripheral HCNS protecting shell but also high-performance ECL of CPB because of a matching band-edge arrangement.

Molybdenum Carbide Nanoparticles Coated into the Graphene Wrapping N-Doped Porous Carbon Microspheres for Highly Efficient Electrocatalytic Hydrogen Evolution Both in Acidic and Alkaline Media.

Molybdenum carbide (MoC) is recognized as an alternative electrocatalyst to noble metal for the hydrogen evolution reaction (HER). Herein, a facile, low cost, and scalable method is provided for the fabrication of MoC-based eletrocatalyst (MoC/G-NCS) by a spray-drying, and followed by annealing. As-prepared MoC/G-NCS electrocatalyst displays that ultrafine MoC nanopartilces are uniformly embedded into graphene wrapping N-doped porous carbon microspheres derived from chitosan.

Mitophagy receptors sense stress signals and couple mitochondrial dynamic machinery for mitochondrial quality control.

Mitochondria are essential organelles for many fundamental cellular processes, including energy production, fatty acid β-oxidation, metabolite synthesis, iron and calcium homeostasis, and programmed cell death. Mitochondrial quality thus influences not only individual cell functions but also whole body metabolism. Dysregulated mitochondrial quality control is closely associated with the progression of aging related diseases, such as cancers and neurodegenerative disorders.

Selective removal of mitochondria via mitophagy: distinct pathways for different mitochondrial stresses.

The efficient and selective elimination of damaged or excessive mitochondria in response to bioenergetic and environmental cues is critical for maintaining a healthy and appropriate population of mitochondria. Mitophagy is considered to be the central mechanism of mitochondrial quality and quantity control. Atg32, a mitophagy receptor in yeast, recruits mitochondria targeted for degradation into the isolation membrane via both direct and indirect interactions with Atg8.

Effect of electro-acupuncture at different acupoints on neuropeptide and somatostatin in rat brain with irritable bowel syndrome.

Objective: To compare the regulatory effects of electro-acupuncture (EA) at acupoints Zusanli (ST36) and Hegu (LI4) on the visceral hyper-sensitivity in the rat model of irritable bowel syndrome (IBS), and to explore the acting targets and specialty of acupoints.

Methods: Except 8 rats of the normal control group, the rest 32 rats were prepared to set up the IBS models. IBS animal model was prepared by enema with acetic acid.

Purification and physicochemical properties of different polysaccharide fractions from the water extract of Boschniakia rossica and their effect on macrophages activation.

Today more and more attentions had been attracted by many nutritionists and pharmacologists on polysaccharides from natural plants or animals due to their significant biological activities. In this research three polysaccharides (BRR-W1, BRR-WA1 and BRR-WA2) were isolated and purified from the water extract of Boschniakia rossica by DEAE Sepharose Fast Flow and Sepharose 6 Fast Flow column chromatography. Chemical and physical characteristics of three polysaccharides were investigated by a combination of chemical and instrumental analysis methods.

Microwave pretreatment can enhance tolerance of wheat seedlings to CdCl2 stress.

In order to determine the role of microwave in cadmium stress tolerance of wheat (Triticum aestivum L.), seeds were exposed to microwave radiation for 0, 5, 10 and 15 s (wavelength 125 mm, power density 126 mW cm(-2), 2450 MHz), and when the seedlings were 7 d old (with one fully expanded leaves), they were treated with 150 μM CdCl(2) solution for 10 d. Changes in a number of physiological and biochemical characteristics were measured and used as indicators of the protective capacity of microwave radiation in this experiment.