New application of a dye-decolorizing peroxidase immobilized on magnetic nanoparticles for efficient simultaneous degradation of two mycotoxins.

Nowadays the enzymatic approaches are the most promising strategies for mycotoxins detoxification in food stuffs. Herein, the dye-decolorizing peroxidase RhDypB from Rhodococcus jostii was studied for its ability to degrade two mycotoxins in both free and the immobilized enzyme forms. This enzyme was recombinantly expressed and purified, while FeO nanoparticles were prepared and modified with chitosan as the immobilization carrier.

Food-Grade Expression of Two Laccases in and Study on Their Enzymatic Degradation Characteristics for Mycotoxins.

Mycotoxin contamination poses substantial health risks to humans and animals. In this study, the two laccases PpLac1 and AoLac2 from and were selected and heterologously expressed in in a food-grade manner to detoxify aflatoxin B (AFB), zearalenone (ZEN), and deoxynivalenol (DON). Both laccases exhibited degradation activity toward these three mycotoxins, while the efficiency of these for DON was relatively low.

A Novel Strategy for Whole-Cell Biotransformation Enabling Simultaneous l-Phenyllactic Acid Production and Coenzyme Regeneration.

l-Phenyllactic acid (l-PLA) is a small molecular organic acid that exhibits a powerful capacity for inhibition against foodborne pathogens. In this work, we developed a new cost-effective and environmentally friendly process for the biosynthesis of l-PLA. This strategy designed a novel whole-cell biotransformation system employing two heterologous enzymes, namely, phenylalanine dehydrogenase (PheDH) and l-hydroxyisocaproate dehydrogenase (l-HicDH).

In Situ Grown 1D/2D Structure of DySiC on SiC for Enhanced Electromagnetic Wave Absorption.

To improve electromagnetic wave (EMW) absorption performance, a novel nano-laminated DySiC coating was successfully in situ coated on the surface of SiC whisker (SiC/DySiC) using a molten salt approach. A labyrinthine three-dimensional (3D) net was constructed by the one-dimensional (1D) SiC coated with the two-dimensional (2D) DySiC layer with a thickness of ~100 nm, which significantly improved the EMW absorption properties of SiC. Compared to pure SiC with the minimum reflection loss (RL) value of -10.

The substrate selectivity of papain-like proteases from human-infecting coronaviruses correlates with innate immune suppression.

Coronaviruses that can infect humans can cause either common colds (HCoV-NL63, HCoV-229E, HCoV-HKU1, and HCoV-OC43) or severe respiratory symptoms (SARS-CoV-2, SARS-CoV, and MERS-CoV). The papain-like proteases (PLPs) of SARS-CoV, SARS-CoV-2, MERS-CoV, and HCoV-NL63 function in viral innate immune evasion and have deubiquitinating (DUB) and deISGylating activities. We identified the PLPs of HCoV-229E, HCoV-HKU1, and HCoV-OC43 and found that their enzymatic properties correlated with their ability to suppress innate immune responses.

Antimicrobial Activity and Antibiofilm Potential of Coenzyme Q against Typhimurium.

Coenzyme Q (CoQ) has anti-inflammatory and anti-tumor effects; however, the antimicrobial and antibiofilm activities of CoQ against serovar Typhimurium are unknown. Thus, we investigated the bacteriostatic and antibiofilm activities, along with the underlying mechanism, of CoQ against . Typhimurium.