Publications by authors named "Huidie Yue"
Hydroxytyrosol, a fine chemical, is widely utilized in food and pharmaceutical industries. In this study, we constructed a pathway to produce hydroxytyrosol by co-expressing tyrosin-phenol lyase (TPL), L-amino acid dehydrogenase (aadL), α-keto acid decarboxylase (KAD), aldehyde reductase (yahK) and glucose dehydrogenase (gdh). We changed combinations between plasmids with different copy numbers and target genes, resulting in 84% increase in hydroxytyrosol production.
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- Plasmid-based microbial systems are important for producing pharmaceuticals, but they usually need antibiotics for stability.
- Researchers created a new strain called A06Δ by relocating an essential gene and linking it with a specific enzyme on the same plasmid, aiming to remove the antibiotic requirement.
- The modified strain A17Δ outperformed A06Δ in producing phenylpyruvic acid (PPA), yielding 4.1 g L compared to 2.1 g L, and maintained plasmid stability for 10 transfers without antibiotics, eventually producing 18.7 g L of PPA in just 14 hours.