[miR-25-3p down-regulates the expression of ADAM10 to inhibit the differentiation of P19 cells into cardiomyocytes by blocking the Notch signaling pathway].

Objective To investigate the effect of miR-25-3p targeting a disintegrin and metalloproteinase 10 (ADAM10) on the differentiation of P19 cells into cardiomyocytes by regulating Notch signaling pathway. Methods P19 cells were induced to differentiate into cardiomyocytes with dimethyl sulfoxide (DMSO) and collected at 0, 5, and 10 days. The mRNA levels of myocardial differentiation markers GATA4, cTnT, atrial natriuretic polypeptide (ANP) and the level of miR-25-3p were detected by real-time quantitative PCR (qRT-PCR).