Spectral and molecular insights into the variations of dissolved organic matter in shallow groundwater impacted by surface water recharge.

Dissolved organic matter (DOM) represents one of the most active elements in aquatic systems, whose fraction is engaged in chemical and biological reactions. However, fluorescence, molecular diversity and variations of DOM in groundwater systems with the alteration of surface water recharge remain unclear. Herein, Excitation-emission matrix (EEM) fluorescence spectroscopy and Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS) combined with principal component coefficients, parallel factor analyses (PARAFAC) with two‒dimensional correlation spectroscopy (2D-COS) were applied in this study.

New insights into pollution source analysis using receptor models: Effects of interaction between heavy metals and DOM on source identification and apportionment in rivers across industrial city.

To effectively control pollution and protect the ecosystem, it is essential to accurately analyze the potential pollution sources of heavy metals (HMs) in rivers. However, the traditional source apportionment methods based on HMs disregard the interaction between HMs and dissolved organic matter (DOM). In this study, data of HMs and DOM was combined for tracing sources and assessing the effect of interaction between HMs and DOM on source apportionment in urbanized rivers that cross urban (URR), industrial (INR), and rural (RUR) regions.

High potential in synergizing the reduction of dissolved organic carbon concentration and carbon dioxide emissions for submerged-vegetation-covered river networks.

Various technologies and projects have been explored and developed for the synergetic control of environmental pollution and carbon emissions in aquatic ecosystems. Planting submerged vegetation in shallow waters was also expected to achieve this purpose. However, the magnitude and mechanism of carbon dioxide (CO) emission affected by submerged vegetation is not clear enough in complex aquatic ecosystems.

Advancing fluorescence tracing with 3D-2D spectral conversion: A mixed culture on microbial degradation mechanisms of DOM from a large-scale watershed.

Fluorescence tracing, known for its precision, rapid application, and cost-effectiveness, faces challenges due to the microbial degradation of dissolved organic matter (DOM) in aquatic environments, altering its original spectral fingerprint. This study conducted a 15-day microcosm experiment to examine the effects of biodegradation on the spectral properties of DOM from various sources: livestock excrement (EXC), urban sewage (URB), industrial wastewater (IND), and riparian topsoil (tDOM). Our findings show that while the spectral structures of DOM from different sources change during 15 days of microbial degradation, these changes do not overlap or interfere with each other.

Protein Kinase R (PKR) as a Novel dsRNA Sensor in Antiviral Innate Immunity.

Protein kinase R (PKR), a key double-stranded RNA (dsRNA)-activated sensor, is pivotal for cellular responses to diverse stimuli. This protocol delineates a comprehensive methodological framework employing single luciferase assays, yeast assays, immunoblot assays, and quantitative PCR (qPCR) to discern and validate PKR activities and their downstream impacts on NF-κB-activating signaling pathways. These methodologies furnish a systematic approach to unraveling the role of PKR as a dsRNA sensor and effector in antiviral innate immunity, enabling in-depth analyses of dsRNA sensor activities.

Critical review on salt tolerance improvement and salt accumulation inhibition strategies of osmotic membrane bioreactors.

The osmotic membrane bioreactor (OMBR) is a novel wastewater treatment and resource recovery technology combining forward osmosis (FO) and membrane bioreactor. It has attracted attention for its low energy consumption and high contaminant removal performance. However, in the long-term operation, OMBR faces the problem of salt accumulation due to high salt rejection and reverse salt flux, which affects microbial activity and contaminants removal efficiency.

Insight into spatial variations of DOM fractions and its interactions with microbial communities of shallow groundwater in a mesoscale lowland river watershed.

Dissolved organic matter (DOM) plays a crucial role in driving biogeochemical processes and determining water quality in shallow groundwater systems, where DOM could be susceptible to dynamic influences of surface water influx. This study employed fluorescence excitation-emission matrix (EEM) spectroscopy combined with principal component coefficients, parallel factor analysis (PARAFAC), co-occurrence network analysis and structural equation modeling (SEM) to examine changes of DOM fractions from surface water to shallow groundwater in a mesoscale lowland river basin. Combining stable isotope and hydrochemical parameters, except for surface water (SW), two groups of groundwater samples were defined, namely, deeply influenced by surface water (IGW) and groundwater nearly non-influenced by surface water (UGW), which were 50.

Mitigated NO emissions from submerged-plant-covered aquatic ecosystems on the Changjiang River Delta.

Submerged plants affect nitrogen cycling in aquatic ecosystems. However, whether and how submerged plants change nitrous oxide (NO) production mechanism and emissions flux remains controversial. Current research primarily focuses on the feedback from NO release to variation of substrate level and microbial communities.

Applying fluorescence spectroscopy coupled with Gaussian band fitting to reveal dynamic variation process of humus fractions from riparian soils along an urbanized river.

Humus, an important fraction of soil organic matter, play an environmental role on nutrients, organic and inorganic pollutants in riparian zones of urbanized rivers. In this study, dynamic variation process of humus fractions from riparian soils was revealed along Puhe River. Composite soil samples of four depths were collected from four land-uses, i.

Appling fluorescence spectroscopy with absolute principal component coefficient to explore dynamic migration of DOM fractions from an urbanized river during torrential rainfall.

This research delves into the dynamic interplay between urbanization and the characteristics of Dissolved Organic Matter (DOM) in the Anyang River, particularly under the stress of torrential rain. The motivation stems from a critical need to decipher how urban landscapes influence water quality, focusing on the intricate transformations and movements of DOM. Employing advanced fluorescence spectroscopy techniques like Excitation-Emission Matrices (EEM) and Parallel Factor Analysis (PARAFAC), the study meticulously differentiates DOM compositions in urban and agricultural settings.

Differences in greenhouse gas emissions and microbial communities between underground and conventionally constructed wastewater treatment plants.

Large quantities of greenhouse gases (GHGs) are emitted into the atmosphere during wastewater treatment. In this study, GHG and microbial samples were collected from four wastewater treatment plants (WWTPs), and their differences and relationships were assessed. The study showed that, compared with conventionally constructed WWTPs, well-established gas collection systems in underground WWTPs facilitate comprehensive collection and accurate accounting of GHGs.

Characterizing dissolved organic matter and bacterial community interactions in a river network under anthropogenic landcover.

Anthropogenic landcover could rise nutrient concentrations and impact the characteristics and bioavailability of dissolved organic matter (DOM) in a river network. Exploring the interactions between DOM and microbials might be conducive to revealing biogeochemistry behaviors of organic matter. In this study, synchronous fluorescence spectra (SFS) with Gaussian band fitting and two-dimensional correlation spectroscopy (2D-COS) were employed to identify DOM fractions and reveal their interactions with bacterial communities.

Applying fluorescence spectroscopy and DNA pyrosequencing with 2D-COS and co-occurrence network to deconstruct dynamical DOM degradation of air-land-water sources in an urban river.

In an urban river, comprehending the interplay between dissolved organic matter (DOM) and atmospheric, terrestrial, and aquatic sources is crucial. This encompassed investigating temporal variations in DOM and its association with the bacterioplankton community to gain profound insights into the biogeochemical dynamics and biodegradability of DOM. DOM was extracted from PM2.

Driving mechanism of water replenishment on DOM composition and eutrophic status changes of lake in arid and semi-arid regions of loess area.

Water replenishment can be a key factor in driving lake eutrophication status. In arid and semi-arid regions of China, water replenishment for a lake has been widely carried out for not only improving water environmental quality, but also maintaining ecological system function. However, it is still unclear in terms of mechanism by which water replenishment drives lake eutrophication status.

A novel recyclable hemoperfusion adsorbent based on TiO nanotube arrays for the selective removal of β-microglobulin.

Prolonged and excessive accumulation of β-microglobulin (βm) in the blood can lead to various kidney-related and other diseases. Currently, the most effective method of removing βm from the blood is hemoperfusion. Although some traditional hemoperfusion adsorbents such as cellulose and polystyrene microspheres have been used for the removal of βm, their selectivity still needs improvement.

Insight into the microbial nitrogen cycle in riparian soils in an agricultural region.

Riparian zones are considered as an effective measure on preventing agricultural non-point source nitrogen (N) pollution. However, the mechanism underlying microbial N removal and the characteristics of N-cycle in riparian soils remain elusive. In this study, we systematically monitored the soil potential nitrification rate (PNR), denitrification potential (DP), as well as net NO production rate, and further used metagenomic sequencing to elucidate the mechanism underlying microbial N removal.

Insight into suppression of dibutyl phthalate on DOM removal during municipal sewage treatment using fluorescence spectroscopy with PARAFAC and moving-window 2D-COS.

Dibutyl phthalate (DBP) has been widely detected in municipal and industrial wastewater, which could indirectly inhibit pollutant removals, especially degradation of dissolved organic matter (DOM). Here, the inhibition of DBP on DOM removal from wastewater in pilot-scale A2O-MBR system was investigated by fluorescence spectroscopy with two-dimensional correlation (2D-COS) and structural equation modeling (SEM). Seven components were extracted from DOM using parallel factor analysis, i.

Explore variations of DOM components in different landcover areas of riparian zone by EEM-PARAFAC and partial least squares structural equation model.

Dissolved organic matter (DOM) plays key roles in species-distribution of contaminants and the biogeochemical cycle of carbon in ecosystems. Riparian zone is the representative of water-land ecotone and controls the DOM exchange between water and land. However, the variance of DOM in different landcover areas of an urban river riparian zone is unknown.

Impacts of dibutyl phthalate on bacterial community composition and carbon and nitrogen metabolic pathways in a municipal wastewater treatment system.

Dibutyl phthalate (DBP) is a typical toxic and hazardous pollutant in pharmaceutical wastewater, affecting the metabolism of microbial flora, leading to decreased treatment efficiency, and deteriorated effluent quality in municipal wastewater treatment plants (WWTPs). This study conducted a long-term experiment with 6 operational stages in a pilot-scale AO-MBR system, analyzing the effect of DBP on the bacterial community and their carbon and nitrogen metabolic pathways. 16S rRNA gene amplicon sequencing analysis and principal components analysis (PCA) showed that DBP at 8 mg/L significantly influenced the structure of bacterial community (P < 0.

Deletion of African Swine Fever Virus (ASFV) H240R Gene Attenuates the Virulence of ASFV by Enhancing NLRP3-Mediated Inflammatory Responses.

African swine fever (ASF) is a highly contagious infectious disease of domestic pigs and wild boars caused by African swine fever virus (ASFV), with a mortality rate of up to 100%. In order to replicate efficiently in macrophages and monocytes, ASFV has evolved multiple strategies to evade host antiviral responses. However, the underlying molecular mechanisms by which ASFV-encoded proteins execute immune evasion are not fully understood.

Molecular Mechanisms of Poxvirus Evolution.

Poxviruses are often thought to evolve relatively slowly because they are double-stranded DNA pathogens with proofreading polymerases. However, poxviruses have highly adaptable genomes and can undergo relatively rapid genotypic and phenotypic change, as illustrated by the recent increase in human-to-human transmission of monkeypox virus. Advances in deep sequencing technologies have demonstrated standing nucleotide variation in poxvirus populations, which has been underappreciated.

Applying synchronous fluorescence spectra with Gaussian band fitting and two-dimensional correlation to characterize structural composition of DOM from soils in an aquatic-terrestrial ecotone.

Dissolved organic matter (DOM), the primary participant of carbon and nitrogen cycle, has a great impact on the behavior and fate of organic pollutants and heavy metals in eutrophic lakes. The dynamic spectral properties of DOM fractions were revealed in an aquatic-terrestrial ecotone under the different types of land use. Composite soil samples of different depths (0-20, 20-40 and 40-60 cm) were collected from four different land uses along a disturbed-impact gradient in Taihu Lake, China, i.

Applying EEM-PARAFAC combined with moving-window 2DCOS and structural equation modeling to characterize binding properties of Cu (II) with DOM from different sources in an urbanized river.

Dissolved organic matter (DOM) in aquatic environment distinctly affects the behavior and fate of heavy metals via complexation, while the interfacial mechanisms and processes are still lacking in detail. Here, Cu (II) binding characteristics of DOM originated from hilly (NDOM), rural (RDOM) and urban (UDOM) regions in an urbanized river was explored by fluorescence excitation-emission matrix spectroscopy (EEM) combined with principal component coefficients, parallel factor analyses (PARAFAC), moving-window two-dimensional correlation spectroscopy (MW2DCOS) and structural equation modeling (SEM). Eight components were extracted from the titrants through EEM-PARAFAC, i.

Impacts of dibutyl phthalate on biological municipal wastewater treatment in a pilot-scale A/O-MBR system.

Dibutyl phthalate (DBP) is a typical contaminant in pharmaceutical wastewater with strong bio-depressive properties which potentially affects the operation of municipal wastewater treatment systems. Based on a year-round monitoring of the quality of influent and effluent of a full-scale pharmaceutical wastewater treatment plant in Northeast China, the DBP was found to be the representative pollutant and its concentration in the effluent ranged 4.28 ± 0.