Bone marrow stromal cell-derived exosome combinate with fibrin on tantalum coating titanium implant accelerates osseointegration.

This study aims to present a sustainably releasing system of exosomes-fibrin combinate loaded on tantalum-coating titanium implants. We hope to investigate potential effects of the system on osseointegration between tantalum coating titanium implants and its surrounding bone tissue. Exosomes derived from rabbit bone marrow stromal cells (rBMSCs) and fibrin were deposited onto the micro-nanostructure tantalum coating surface (Ta + exo + FI) and compared to control groups, including tantalum coating (Ta), tantalum coating loaded exosomes (Ta + exo) and tantalum coating loaded fibrin (Ta + FI).

Primary Extra Nodal Diffuse Large B-Cell Lymphoma of the Maxillary Sinus with Symptoms of Acute Pulpitis.

Diffuse large B-cell lymphoma not otherwise specified (DLBCL-NOS) is a subtype of large B-cell non-Hodgkin lymphoma with various clinical and pathological manifestations. DLBCL-NOS which primarily arises from maxillary sinus is rare and hard to diagnose due to unique anatomy. Here, we present a case of DLBCL-NOS that developed in the left maxillary sinus of a 72-year-old male, who presented with severe toothache that resembled acute pulpitis.

The transition from invariant to action-dependent visual object representation in human dorsal pathway.

The human brain can efficiently process action-related visual information, which supports our ability to quickly understand and learn others' actions. The visual information of goal-directed action is extensively represented in the parietal and frontal cortex, but how actions and goal-objects are represented within this neural network is not fully understood. Specifically, which part of this dorsal network represents the identity of goal-objects? Is such goal-object information encoded at an abstract level or highly interactive with action representations? Here, we used functional magnetic resonance imaging with a large number of participants (n = 94) to investigate the neural representation of goal-objects and actions when participants viewed goal-directed action videos.

Microbial transformation of oleanolic acid by Trichothecium roseum.

Microbial transformation of the oleanolic acid (1) using Trichothecium roseum (pers.) Link (M 95.56) has resulted in the isolation of two new hydroxylated type metabolites, characterized as 15α-hydroxy-3-oxo-olean-12-en-28-oic acid (2) and 7β,15α-dihydroxy-3-oxo-olean-12-en-28-oic acid (3).

[Experiment on inducing human periodontal ligament stem cells into adipose cells].

Objective: To explore the capability of human periodontal ligament stem cells (PDLSCs) differentiating into adipose cells in vitro and to determine their changes in cell morphology, structure and function during differentiation.

Methods: PDLSCs isolated by magnetic-activated cell selection were treated continuously with adipogenic medium for 21 d. Then the cell morphology, ultrastructure, adipose specific markers of low density lipoprotein (LPL) and peroxisome proliferator activated receptor-gamma (PPAR-gamma) were analyzed by inverted contrast microscope, trans mission electron microscope (TEM), flow cytometry, immunofluorescence, RT-PCR and Western blot, respectively.

[Optimization on the production of analgesic peptide from Buthus martensii Karsch in Pichia pastoris].

The technology of liquid fermentation for producing the recombinant analgesic peptide BmK AngM1 from Buthus martensii Karsch in Pichia pastoris was studied by single-factor and orthogonal test. The results showed that the optimal culture conditions were as follows: 1.2% methanol, 0.

[Isolation and identification of dog periodontal ligament stem cells].

Objective: To isolate, culture and identify a dog periodontal ligament stem cells (PDLSC) line in vitro.

Methods: The adult dog periodontal ligament cells were isolated by limited dilution of culture cell for single cell clone. Cells originated from one of these clones were assessed through colony-forming efficiency and immunocytochemistry assay and alkaline phosphatase stain was used to identify the source of adult dog periodontal stem cells, at the same time, PDLSC were induced with mineralizatin solution and was found to have long protrude like an osteoblast.

[Molecular phylogenetic analysis of Paecilomyces hepiali and Cordyceps sinensis].

Phylogenetic relationship between Paecilomyces hepiali and Cordyceps sinensis was studied by analyzing the sequence of rDNA-ITS. The samples of C. sinensis were collected from Hualong County in Qinghai Province and Kangding County in Sichuan Province in May and June, respectively.

Microbial O-demethylation, hydroxylation, sulfation, and ribosylation of a xanthone derivative from Halenia elliptica.

1-Hydroxy-2,3,5-trimethoxyxanthone (1), one of the major xanthone derivatives isolated from Halenia elliptica, was biotransformed by two fungi, Trichothecium roseum and Paecilomyces marquandii. Transformation of 1 by T. roseumgave 1,5-dihydroxy-2,3-dimethoxyxanthone (2), 5-O-sulfate-1-hydroxy-2,3-dimethoxyxanthone (3), 5-O-sulfate-1-hydroxy-2,3,7-trimethoxyxanthone (4), 5-O-beta-ribofuranosyl-1-hydroxy-2,3-dimethoxyxanthone (5), and 1,5,6-trihydroxy-2,3-dimethoxyxanthone (6).

[Correlations between the characters of the mycelium vegetative growth and the formation of the fruiting body of Ganoderma luciderm].

Objective: To select a proper Ganoderma luciderm strain for the fruiting body production.

Method: The strains were cultivated on the agar media and in the liquid media, respectively. Then the strains were inoculated onto the solid medium made from agricultural products (such as wheat bran, corn powder, wood meal, etc.

Molecular cloning, expression and characterization of hyoscyamine 6beta-hydroxylase from hairy roots of Anisodus tanguticus.

Anisodus tanguticus, one of the indigenous Chinese ethnological medicinal plants of the Solanaceae, produces anticholinergic alkaloids such as hyoscyamine, 6 beta-hydroxyhyoscyamine and scopolamine. Hyoscyamine 6 beta-hydroxylase (H6H), a key enzyme in the biosynthetic pathway of scopolamine, catalyzes the hydroxylation of hyoscyamine and epoxide formation from 6 beta-hydroxyhyoscyamine to generate scopolamine. A full-length cDNA of H6H has been isolated from A.

[Biotransformation of dehydroepiandrosterone by hairy root cultures of Anisodus tanguticus].

Aim: To modify the structure of dehydroepiandrosterone (DHEA).

Methods: Using hairy root cultures of Anisodus tanguticus to perform biotransformation of DHEA, using chromatographic and spectral techniques to isolate and identify the products.

Results: (1) The MS medium without plant hormone was suitable for the growth of the hairy root.