Publications by authors named "Hui-ting Qu"
Article Synopsis
- Immune thrombocytopenic purpura (ITP) is an autoimmune condition affecting platelet levels, and this study focused on newly diagnosed patients to identify genetic variants linked to the disease.
- Researchers utilized whole-exome sequencing on bone marrow samples from 20 active ITP patients to analyze the genetic makeup and determined the involvement of 3998 missense mutations across 2269 genes.
- Key findings revealed specific genetic variants significantly related to ITP, with most mutations impacting crucial signaling pathways, highlighting the need for further research to fully understand the genetic factors at play.*
Background: Immune thrombocytopenia (ITP) is a prevalent autoimmune disease with a complex aetiology where DNA methylation changes are becoming triggers.
Method: To investigate novel abnormally methylated genes in the pathogenesis of ITP, we performed a high-throughput methylation analysis on 21 ITP patients and 9 normal control samples. We analysed the extent of key methylated genes and their downstream cytokines through Luminex assay or qRT-PCR.
Purpose: In view of the numerous clinical observations and laboratory studies that suggest a critical role for the spleen in immune thrombocytopenia (ITP) pathophysiology, we aimed to characterize Th1-associated chemokine receptors CXCR3 and CCR5 and Th2-associated chemokine receptor CCR3 in spleens of ITP patients and assess the significance of their differential expression in the clinical setting.
Methods: The histopathology of spleens was observed using hematoxylin-eosin staining (HE), and the positive rate of CXCR3, CCR5 and CCR3 expression in spleens of 24 ITP patients and 12 patients with traumatic splenic rupture as normal controls was detected by immunohistochemistry using the SP method. CXCR3, CCR5 and CCR3 protein expression was analyzed by Western blot and mRNA levels were investigated by real-time polymerase chain reaction (RT-PCR).
[Expression of CXCR3 and CCR5 chemokine receptor in spleens of patients with primary immune thrombocytopenia].
Zhonghua Xue Ye Xue Za Zhi
November 2012
Objective: To study CXCR3 and CCR5 chemokine receptor expression in spleens of patients with primary immune thrombocytopenia (ITP) and its clinical significance.
Methods: The splenectomy specimens from 10 ITP patients (ITP group) and 8 patients with traumatic splenic rupture (normal control group) were studied. Immunohistochemistry (IHC) was used to study the positive rate of CXCR3 and CCR5.