Plasma metabolomic profiling of proliferative diabetic retinopathy.

Background: Proliferative diabetic retinopathy (PDR), a sight-threatening retinopathy, is the leading cause of irreversible blindness in adults. Despite strict control of systemic risk factors, a fraction of patients with diabetes develop PDR, suggesting the existence of other potential pathogenic factors underlying PDR. This study aimed to investigate the plasma metabotype of patients with PDR and to identify novel metabolite markers for PDR.

[Expression and significance of Shh and Wnt5a genes in Cornelia de Lange syndrome].

Objective: To study the expression of Shh and Wnt5a genes in the limb buds of NIPBL fetal rats and the association of these two genes with Cornelia de Lange syndrome (CdLS).

Methods: A total of 72 NIPBL fetal rats were divided into an experimental group and a control group, with 36 rats in each group. The limb buds were collected from 12 fetal rats each on embryonic days 10, 11 and 12 (E10, E11 and E12) respectively.

Risk of silent myocardial ischemia detected by single photon emission computed tomography (SPECT) among asymptomatic Chinese patients with type 2 diabetes.

To explore the prevalence and risk factors of silent myocardial ischemia (SMI) detected by using single photon emission computed tomography (SPECT) in Chinese asymptomatic patients with type 2 diabetes (T2D).In this hospital-based retrospective study, 821 T2D patients who were screened for SMI detected by stress myocardial perfusion imaging using SPECT between June 2014 and July 2016 were investigated. Clinical indicators were compared between the patients with SMI and controls without SMI.

[The receptor-dependent and non-receptor-dependent mechanism of melatonin activated BK channels in middle cerebral artery myocyte].

Objective: To investigate the mechanisms through which myocyte large-conductance Ca-activated K (BK) channels mediate the vasodilation effects of melatonin on cerebral arteries (CAs).

Methods: Middle cerebral arteries (MCA) were obtained from 8-week-old male Wistar rats after anaesthetized. Middle cerebral arterial smooth muscle cells were enzymatically isolated.

[Relationship between soil bacterial community and environmental factors in the degraded alpine grassland of eastern Qilian Mountains, China].

To explore the distribution of bacterial community and its relationship with soil environmental factors in degraded alpine grasslands in the eastern Qilian Mountains, we analyzed the changes of bacterial community structure and diversity across lightly, moderately and severely degraded grasslands by using high-throughput sequencing technology. Redundancy analysis (RDA) was performed to analyze the relationship between soil bacterial communities and soil environmental factors by using CANOCO 4.5 software.

[Fabrication and accuracy research on 3D printing dental model based on cone beam computed tomography digital modeling].

Objective: The aim of this study is to build a digital dental model with cone beam computed tomography (CBCT), to fabricate a virtual model via 3D printing, and to determine the accuracy of 3D printing dental model by comparing the result with a traditional dental cast.

Methods: CBCT of orthodontic patients was obtained to build a digital dental model by using Mimics 10.01 and Geomagic studio software.

[Association between high-mobility group box 1 and neonatal respiratory distress syndrome].

Objective: To study the association between serum level of high-mobility group box 1(HMGB1) and neonatal respiratory distress syndrome (NRDS).

Methods: A total of 35 infants with NRDS and 35 normal neonates (control group) were enrolled. Peripheral venous blood samples were collected with 12-24 hours after birth.

[Diagnostic values of urinary netrin-1 and kidney injury molecule-1 for acute kidney injury induced by neonatal asphyxia].

Objective: To investigate the values of urinary netrin-1 and kidney injury molecule-1 (KIM-1) in the early diagnosis of acute kidney injury (AKI) induced by neonatal asphyxia.

Methods: A total of 80 full-term neonates with asphyxia were enrolled (mild asphyxia: 34 neonates; severe asphyxia: 46 neonates). Forty normal full-term neonates were selected as the control group.

Identification of Salt-Stress-Induced Genes from the RNA-Seq Data of Reaumuria trigyna Using Differential-Display Reverse Transcription PCR.

Next generation sequencing (NGS) technologies have been used to generate huge amounts of sequencing data from many organisms. However, the correct choice of candidate genes and prevention of false-positive results computed from digital gene expression (DGE) of RNA-seq data are vital when using these genetic resources. We indirectly identified 18 salt-stress-induced Reaumuria trigyna transcripts from the transcriptome sequencing data using differential-display reverse transcription PCR (DDRT-PCR) combined with local BLAST searches.

Cloning and expression of a novel antifreeze protein AFP72 from the beetle Tenebrio molitor.

A novel antifreeze protein AFP72 cDNA (GenBbank accession No. AY929389) was obtained by RT-PCR from Tenebrio molitor. The 216 bp fragment encodes a protein of 72 amino acid residues.

[Clinical features and carotid artery color Doppler imaging in patients with ocular ischemic syndrome].

Objective: To report the clinical manifestations and results of carotid artery color Doppler imaging (CDI) of ocular ischemic syndrome (OIS).

Methods: It was a retrospective case series study. Consecutive patients suspected with OIS were retrospectively studied from 1995 to 2009.

[Clinical characteristics of choroidal metastasis].

Objective: To study the clinical characteristics of choroidal metastasis (CM) to promote the early diagnosis and differentiate from other choroidal tumors.

Methods: Retrospective clinical observational cases. All patients with choroidal metastasis underwent ophthalmologic examination including best corrected visual acuity (VA), slit-lamp examination, binocular indirect funduscopy, color photography, fundus fluorescein angiography (FFA), indocyanine-green angiography (ICGA), optical coherence tomography (OCT), A and B scan ultrasound examination, magnetic resonance image (MRI) as well as CT of the thorax, etc.

[The prevalence and risk factors for coronary stenosis in patients with cerebral infarction].

Objective: To study the morbidity rate of and relevance to coronary stenosis in cerebral infarction patients.

Methods: CT coronary angiography was performed in 112 cases of cerebral infarction after CT cerebral angiography. Multivariate logistic regression analysis was carried out between the clinical data and coronary stenosis.

[Clinical manifestations of retinal artery macroaneurysms].

Objective: To summarize the clinical characteristics of retinal arterial macroaneurysm and to improve the diagnosis accuracy in order to differentiate it from other fundus diseases.

Methods: It was a retrospective case series.

Results: of fluorescein angiography or indocyanine angiography, associated with ultrasound, OCT and several other examinations were analyzed in 22 patients (22 eyes) with retinal arterial macroaneurysm.

(E)-Methyl 3-{3-[(4-bromo-phen-oxy)-methyl]phen-yl}acrylate.

In the mol-ecule of the title compound, C(17)H(15)BrO(3), the rings make a dihedral angle of 75.54 (17)°. In the crystal structure, inter-molecular C-H⋯O hydrogen bonds link the mol-ecules into centrosymmetric dimers, and the π-stacked dimers inter-act with neighbouring dimers via C-H⋯π stacking inter-actions.

[Discussion of several problems in ophthalmic research in China].

Based on the manuscripts received by several ophthalmologic journals, major issues regarding current ophthalmic research aspects in China were analyzed and discussed, including the selection of project, the design of research, the choice of statistical treatments and the techniques of writing research manuscripts. This article provides suggestions for Chinese ophthalmologists to improve the quality of their research work.

[Measurement of macular edema in retinal vein occlusion using optical coherence tomography and its visual prognosis].

Objective: To examine the type of macular edema in patients with retinal vein occlusion by using optical coherence tomography (OCT). To compare the sensitivity and specificity between OCT and fundus fluorescein angiography (FFA). To investigate the visual prognosis and risk factors in patients with cystoid macular edema (CME).

[The occurrence of actual F factor transfer during the stationary-phase mutation in Escherichia coli FC40].

The experiment of adaptive mutation was performed by using Escherichia coli GM133 rif(r) as test cells and HB214 str(r) as scavenger cells. Transfer frequency between GM133 and HB214 was estimated,based on the number of revertants appeared on the selective plates when GM133 were killed by addition of M9 selective medium containing 100 microg/mL of streptomycin at different time. After 30 minutes the cells of GM133 and HB214 were mixed,the estimated transfer frequency was about 0.

[The inhibitory effect of 8-chloroadenosine on tumor necrosis factor-induced proto-oncogene expression of retinal pigment epithelial cells].

Objective: To investigate the inhibitory effect of 8-chloroadenodine (8-CLA) on tumor necrosis factor-alpha (TNF-alpha)-induced proto-oncogene expression in retinal pigment epithelial (RPE) cells.

Methods: Primary culture and subculture of health bovine RPE cells were established in vitro. RPE cells were treated with tumor necrosis factor TNF-alpha (13.

[Inhibition of lens epithelial cell cycle and PCNA mRNA expression by an antisense oligonucleotides complementary to the messenger RNA of proliferating cell nuclear antigen].

Objective: To determine whether antisense oligonucleotides complementary to the messenger RNA of proliferating cell nuclear antigen (PCNA ASODN) inhibit the proliferation of bovine lens epithelial cell (BLEC) by changing the cell cycle and down-regulating the expression of PCNA mRNA and PCNA protein.

Methods: BLECs were cultured in vitro, and the second passage cells were used in this experiment. PCNA ASODN (30 micro mol/L), PCNA SODN (sense oligonucleotides, 30 micro mol/L), basic fibroblast growth factor (bFGF, 10 micro g/L), bFGF (10 ng/ml) + ASODN (30 micro mol/L), bFGF (10 micro g/L) + SODN (30 micro mol/L) were introduced respectively into the medium, and the same amount of PBS was added into the medium as a control.