Dynamic changes in plasma tissue plasminogen activator, plasminogen activator inhibitor-1 and beta-thromboglobulin content in ischemic stroke.

The aim of this paper is to investigate the corresponding variations of plasma tissue plasminogen activator (t-PA) and plasminogen activator inhibitor-1 (PAI-1) activities, and beta-thromboglobulin (β-TG) content in patients during different stages of ischemic stroke. Ischemic stroke is a common disease among aging people and its occurrence is associated with abnormalities in the fibrinolytic system and platelet function. However, few reports focus on the dynamic changes in the plasma fibrinolytic system and β-TG content in patients with ischemic stroke.

A novel spectrophotometric method for indirect determination of nitric oxide (NO) in serum.

Background: It is essential in clinical medicine to develop a simple and accurate analytical method for detecting nitric oxide (NO) in blood or other body fluid. In order to overcome the shortcomings of previous methods, this experiment developed a novel indirect analytical method of NO in serum.

Methods: After nitrate was reduced by copper-coated cadmium granules, the nitrite produced was determined by diazotization of fuchsin acid and coupling to resorcinol.

Simultaneous determination of l-citrulline and l-arginine in plasma by high performance liquid chromatography.

Objective: To develop a method for simultaneously determining l-citrulline and L-arginine levels in plasma using RP-HPLC with ultraviolet detection.

Design And Methods: Plasma samples were deproteinized by trichloroacetic acid and heat. Phenyl-isothiocyanate (PITC) solution was used as derivatization reagent and a gradient elution was carried out.