[Establishment and application of TaqMan Real-time PCR for the detection of pathogenic Leptospira species].

Objective: To develop and evaluate a TaqMan Real-time PCR method for the detection of pathogenic Leptospira species.

Methods: rrs gene of part fragment on 16S rRNA was used to design primers and TaqMan probe. The target gene was cloned into vector pMD19-T in order to make the standard curve and be used for quality control.