Publications by authors named "Hui-ling Guo"

Phosphorus (P) is an essential nutrient for crop growth, making it important for maintaining food security as the global population continues to increase. Plants acquire P primarily via the uptake of inorganic phosphate (Pi) in soil through their roots. Pi, which is usually sequestered in soils, is not easily absorbed by plants and represses plant growth.

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  • - The study investigates a new type of carbon dot, specifically nitrogen-doped negatively charged carbon dots (TPP-CDs), designed to combat bacterial wound infections without causing resistance, while being biocompatible for human cells.
  • - TPP-CDs are created using tannic acid, polyethylene polyamine, and polyethylene glycol as ingredients, and they effectively destroy bacterial biofilms and DNA, while inducing oxidative stress for enhanced antimicrobial effects.
  • - Additionally, TPP-CDs improve wound healing by promoting cell growth, reducing inflammation, and scavenging free radicals, indicating their potential for practical medical applications in infection management.
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Introduction: The nursing management of intracranial hypertension in adult patients with severe brain injury is crucial for maintaining the stability of intracranial pressure, which ultimately improves patient outcomes.

Objectives: This project aimed to implement evidence-based practices for the nursing management of intracranial hypertension in adult patients with severe brain injury.

Methods: This evidence implementation project was conducted in a neurosurgery intensive care unit in a large tertiary hospital in Guangzhou, China.

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As an essential macronutrient, phosphorus (P) is often a limiting nutrient because of its low availability and mobility in soils. Drought is a major environmental stress that reduces crop yield. How plants balance and combine P-starvation responses (PSRs) and drought resistance is unclear.

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  • Bone metastasis (BM) significantly worsens the prognosis for patients with primary malignant bone neoplasms (PMBNs), with only 4.8% of diagnosed patients having BM at diagnosis.
  • Key risk factors for developing BM include tumor location, size, type (notably Ewing sarcoma), and regional lymph node involvement.
  • A nomogram created from identified prognostic factors (like tumor type and treatment history) can effectively predict cancer-specific survival for these patients.
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Since leaves are rich in bioactive constitutes, particularly volatile compounds, there are higher demands for high-quality dried medicinal and aromatic peppermint products. This study aimed to assess the drying kinetics of hot air thin layer drying leaves and exploring the effects of hot air-drying temperatures on the textural properties and sensory quality. According to our results, the Midilli model is the best model representing the hot air-drying process.

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The present study explored the kinetics and variation of volatile components of Atractylodis Macrocephalae Rhizoma during the hot-air drying process to obtain the optimal process parameters under multiple goals such as drying efficiency and drying quality. The dry basis moisture content and drying rate curves along with the change of drying time of Atractylodis Macrocephalae Rhizoma were investigated at five levels of drying air temperatures(30, 40, 50, 60, and 70 ℃). The relationship between moisture ratio and time in the drying process of Atractylodis Macrocephalae Rhizoma was fitted and verified by Midilli model, Page model, Overhults model, Modified Page model, Logaritmic model, Two terms Exponential model, and Newton model.

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Background: Through previous studies and clinical practice, we have found that real-time ultrasound-guided (UG) spinal anesthesia (SA) and traditional landmark-guided (LG) SA each require a different minimum local anesthetic dose (MLAD) of ropivacaine. For this study, we used Dixon's up-and-down sequential method to analyze and compare the MLAD of different ropivacaine concentrations required for the UG and LG SA methods.

Methods: A total of 120 patients undergoing knee surgery were consecutively recruited and randomly divided into four groups (30 patients per group).

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Phylogeny of hard ticks (Ixodidae) remains unresolved. Mitochondrial genomes (mitogenomes) are increasingly used to resolve phylogenetic controversies, but remain unavailable for the entire large Hyalomma genus. Hyalomma asiaticum is a parasitic tick distributed throughout the Asia.

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Background: Truth-telling toward terminally ill patients is a challenging ethical issue in healthcare practice. However, there are no existing ethical guidelines or frameworks provided for Chinese nurses in relation to decision-making on truth-telling of terminal illness and the role of nurses thus is not explicit when encountering this issue.

Objectives: The intention of this paper is to provide ethical guidelines or strategies with regards to decision-making on truth-telling of terminal illness for Chinese nurses.

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We conducted a serological survey to detect antibodies against avian influenza virus (AIV) in Gazella subgutturosa, Canis lupus, Capreolus pygargus, Sus scrofa, Cervus elaphus, Capra ibex, Ovis ammon, Bos grunniens and Pseudois nayaur in Xinjiang, China. Two hundred forty-six sera collected from 2009 to 2013 were assayed for antibodies against H5, H7 and H9 AIVs using hemagglutination inhibition (HI) tests and a pan-influenza competitive ELISA. Across all tested wildlife species, 4.

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Objective: To investigate the effect of Foxp3 gene modified dendritic cells (Foxp3 + DC) on allogeneic T cells proliferation and to study the effect of Foxp3 + DC on corneal allograft rejection.

Methods: Lentivirus-Foxp3 was transfected into DC2.4 cells, as Foxp3 + DC cells.

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Electrospun fiber mesh has been a candidate for guided bone regeneration membrane. However, its poor mechanics property has been limited in clinical application. In this study, various star-shaped poly(ε-caprolactones) (PCLs) are successfully synthesized by ring-opening polymerization and mixed with poly(l-lactide) (PLLA) to be made into blended membranes through electrospinning.

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Objective: This study is conducted to evaluate the effects of anti-HER-2 anti-CD3 bi-specific antibodies(BsAb) on HER-2/neuover-expressing human colorectal carcinoma cells.

Methods: Growth was assessed by MTT assays after exposure of HCT-116 cells to Herceptin, anti-CD3 and BsAb antibodies. Immunocytochemistry was applied to test the HER-2 level of HCT-116.

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Insulin-stimulated glucose uptake by the glucose transporter GLUT4 plays a central role in whole-body glucose homeostasis, dysregulation of which leads to type 2 diabetes. However, the molecular components and mechanisms regulating insulin-stimulated glucose uptake remain largely unclear. Here, we demonstrate that Axin interacts with the ADP-ribosylase tankyrase 2 (TNKS2) and the kinesin motor protein KIF3A, forming a ternary complex crucial for GLUT4 translocation in response to insulin.

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Objective: To study the cell membrane of corneal endothelium with a micromolecular compound J2 in corneal allograft of rat using atomic force microscopy (AFM).

Methods: Cohort study. Subjects were divided into two groups: group A (n = 15): experimental group; group B (n = 15): placebo control group.

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  • The study aimed to analyze changes in the vault of implantable Collamer lenses (ICL) and their impact on visual outcomes one year after implantation.
  • 127 eyes from 66 patients were examined before and after the surgery, focusing on factors like diameter, vault, refractive error, and wavefront aberrations.
  • Results showed a significant decrease in vault within the first month, stabilizing afterward, but this change did not notably affect visual outcomes after a year.*
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  • * Common methods like chromatography and immunoassays are widely used but often yield one-dimensional results and can be complicated due to various interferences.
  • * Non-invasive methods like imaging and Raman spectroscopy offer continuous in vivo analysis for specific drugs, though they have limitations in sensitivity and specificity, highlighting the need for a careful choice of methods based on the drug's characteristics.
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  • The study aimed to investigate the effects of different dosages of N(2)O(4) on the multifocal electroretinogram (ERG) in mice, laying the groundwork for future research.
  • Normal Wistar mice were injected with various dosages of N(2)O(4), and their multifocal ERG was recorded in the evening after the injection.
  • Results showed variations in the latency of the "b" wave between different groups, while there were no significant differences in the amplitude, suggesting that further studies could build on these findings to explore the effects of N(2)O(4) more deeply.
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Objective: To study the effects of mPer1 gene on the response of mammary carcinoma EMT6 cells to Adriamycin in vitro.

Methods: The eukaryotic expression vector pcDNA3. 1 (+)-mPer1 was transfected into the EMT6 cells (EMT6-mPerl).

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Aim: To study enhance effect of huangqi and dangshen extraction (Shenqi) on pacilitaxel inhibitory metastasis and angiogenesis on mouse Lewis lung carcinoma model.

Methods: Lewis lung carcinoma cells were inoculated into right hind footpad of C57BL/6 mice. Six hour after tumor inoculated, the mice were randomly divided into 3 groups.

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  • The study aimed to investigate how silencing the survivin gene using RNA interference (RNAi) affects the sensitivity of HeLa cervical cancer cells to radiation and the chemotherapy drug cisplatin.
  • HeLa cells were transfected with a plasmid containing survivin-specific siRNA, leading to a significant decrease in survivin gene expression, increased activities of the apoptosis marker caspase-3, and higher rates of cell death.
  • Results showed that silencing survivin not only improved the cancer cells' response to radiation but also made them more susceptible to cisplatin, indicating a potential strategy for enhancing cancer treatment effectiveness.
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  • The study aimed to enhance the effectiveness of an anticancer drug, adriamycin, for treating tongue squamous cell carcinoma in rabbits.
  • Thirty rabbits were divided into two groups, with one group receiving standard adriamycin and the other receiving adriamycin loaded in nanoparticles, administered through a precise artery.
  • Results showed that the nanoparticle method significantly increased the drug concentration in the tumor while reducing it in other organs, suggesting a more targeted treatment approach.
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Objective: To investigate the expression of A-type atrial natriuretic peptide receptor (ANPR-A) in the kidneys of renovascular hypertension rats and evaluate the significance of the expression.

Methods: The rat model of renovascular hypertension was produced by constricting one lateral renal artery. After the renal artery being constricted for 4 weeks and 8 weeks, the systolic BP of rats was measured with a manometer using the tail-cuff method.

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  • The study aimed to investigate how insulin affects the process of apoptosis (cell death) in cultured human trophoblast cells, which are important for pregnancy.
  • Human trophoblast cells were exposed to H2O2 to induce apoptosis, with three groups: a control group, a group treated with H2O2 only, and a group treated with both insulin and H2O2.
  • The results showed that insulin significantly reduced the apoptosis rate caused by H2O2, potentially by lowering caspase-3 activity and increasing Bcl-2 protein levels, suggesting it has a protective effect on these cells.
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