[Comparison between recombinant virus assay and live virus assay on evaluating anti-HIV-1 drugs].

Objective: To compaire results of recombinant virus assay and live virus assay on evaluateing anti-HIV-1 drugs.

Methods: The pseudoviruse was generated by cotransfection of the plasmid B01 containing gp160 genes and pSG3 delta env plasmid. After co-incubation of pseudovirus with serially diluted drug, the EC50 and ED50 were calculated according to RLU(relative light unit) for each drug.

[The impact of the HIV-1 envelope (Env) mutation on its assembly of functional pseudovirus].

To find out whether the mutations of HIV-1 Env have influence on the assembly of pseudovirus and their abilities to infect cells, site-directed mutation (A457D)was performed using cycling mutagenesis and selection of mutants with DpnI. Transformation and plasmid purification technologies were used to obtain mutated env clone. Then both the prototype and the mutant were co-transfected with pSG3(delta(env)) to 293FT cells, respectively.