[Mandibular-driven simultaneous maxillo-mandibular distraction for hemifacial microsomia with rapid prototyping technology].

Objective: To explore the clinical application of mandibular-driven simultaneous maxillo-mandihular distraction to correct hemifacial microsomia with rapid prototyping technology.

Methods: The patient' s skull resin model was manufactured with rapid prototyping technology. The osteotomy was designed on skull resin model.

[Effects of hyaluronic acid on biomechanic performance of porcine acellular dermal matrix plus thin skin autograft after transplantation].

Objective: To explore the effects of hyaluronic acid (HA) on biomechanical properties for porcine acellular dermal matrix (PADM) plus thin skin autograft after transplantation.

Methods: The dorsa of 10 Japanese white rabbits were symmetrically divided into four areas of A-D by random grouping. Full-thickness skin defects were created in Groups A-C while Group D was blank with normal skin.

[Experimental study of hyaluronic acid on the biomechanical compliance of porcine acellular dermal transplantation].

Objective: To explore the effect on biomechanical compliance after an addition of hyaluronic acid (HA) in transplantation of porcine acellular dermal matrix (PADM) with skin autograft.

Methods: Ten Japanese white rabbits were used as experimental animal, the dorsa of rabbits was symmetrically divided into four area, and then grouping was randomized. Full-thickness skin defects were created in Group A to Group C, and Group D was blank group with normal skin.

Influence of hyaluronic acid on wound healing using composite porcine acellular dermal matrix grafts and autologous skin in rabbits.

This study aims to explore the influence of hyaluronic acid (HA) on wound healing during xenogeneic porcine acellular dermal matrix (PADM) composite skin grafting. The results will facilitate the development of methods for improving graft contracture and poor elasticity of composite transplantation. Exogenous HA was added to composite PADM grafts and to thin autologous skin grafts during rabbit full-thickness skin wound repair.

[Effect of PRX-2 gene transferred by lipofectamine on the proliferation of human skin fibroblasts].

Objective: To explore the effects of PRX-2 gene transferred by lipofectamine on the human skin fibroblasts.

Methods: Normal human skin fibroblasts were cultured and PRX-2 gene was transferred by lipofectamine. The proliferation of fibroblasts was detected by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) and flow cytometry.

[Effect of different concentration of tamoxifen ointment on the expression of TGF-beta2 of hypertrophic scar at rabbit ears].

Objective: To observe the effect of different concentration of Tamoxifen ointment on the fibroblasts and transforming growth factor (TGF-beta2) of hypertrophic scar at rabbit ears, so as to explore the possibility of treatment of hypertrophic scar with Tamoxifen.

Methods: The hypertrophic scar model was established in 96 New Zealand rabbits' ears. The wounds were divided into four groups (A, B, C and D), with 144 wounds in each group.

[Effects on collagen I and III after transplantation of porcine acellular dermal matrix with hyaluronic acid].

Objective: To investigate the changes of collagens I and III after the addition of hyaluronic acid in the transplantation of porcine acellular dermal matrix.

Methods: Full-thickness skin defects were created on the dorsa of Japanese white rabbits. And the rabbits were divided randomly into 3 groups: Group A (hyaluronic acid, porcine acellular dermal matrix plus thin skin autografts), Group B (porcine acellular dermal matrix plus thin skin autografts) and Group C (skin autografts).

[An ultrastructure study on the palatomaxillary suture of dog expanded by NiTi-SMA].

Objective: To explore the ultrastructure of the palate-maxillary sutures under tensile forces by transmission electron microscope (TEM).

Methods: The Suture expanders were made in NiTi-Shape memory alloy (NiTi-SMA). The maximum force was 3.

[Application of thoracic skin flap with multiple blood supply in repair of tissue defects and deformities in jaw and neck].

Objective: To explore an appropriate measure to repair tissue defects and deformities in mandibulo-cervical region.

Methods: Eighteen cases with severe tissue defects and deformity in jaw and neck were repaired with thoracic skin flap with multiple blood supply system in our unit from Jan. 2006 to Nov.

Treatment strategies for mass burn casualties.

Background: Mass burn casualties are always a great challenge to a medical team because a large number of seriously injured patients were sent in within a short time. Usually a high mortality is impending. Experiences gained from successful treatment of the victims may be useful in guiding the care of mass casualties in an armed conflict.

[Repairing of lower eyelid ectropion with expanded flap].

Objective: To investigate the effect of reconstruction of lower eyelid ectropion with expanded flap.

Methods: Fourty patients with lower eyelid ectropion were reconstructed using tissue expander. The volume of the smallest expander was 30 ml, and that of the biggest one was 150 ml.

[Differential display of homeobox gene expressions in the normal, wounded human fetal and adult skins by DNA microarray].

Objective: To explore the differential expression of homeobox genes in the normal, wounded human fetal and adult skins and its significance in fetal scarless healing.

Methods: Gene chips containing 14 000 human genes were used to investigate homeobox gene expressions of the normal, wounded human fetal and adult skins.

Results: There were significant differences between the expression of homeobox genes, especially for PRX-2, HOXB13, HOXB6 and HOXB7.

[The role of tyrosine phosphorylation proteins in procollagen gene expression of fibroblasts in wound healing].

Objective: To investigate the role of adhesion between fibronectin and fibroblasts in wound healing as well as tyrosine phosphorylation proteins in procollagen mRNA expression.

Methods: The level of proalpha1 (I) mRNA and tyrosine phosphorylation protein were detected employing the techniques of RT-PCR and immunoblotting. After inhibition of tyrosine kinases, herbimycin A was added to the medium to block the pathway of tyrosine phosphorylation, the changes of procollagen mRNA and tyrosine phosphorylation proteins were further investigated.

[The treatment of deformity of axillary scar contracture after burns].

Objective: To explore the best methods to repair the deformity of axillary scar contracture after burns.

Methods: Ninety cases in 78 patients with axillary scar contracture after burns from January 1998 to January 2002 were analyzed. According to the severity of the deformity and its influence on the function of the shoulder joint.

[A comparative study of PDGF and EGF expression in skin wound healing between human fetal and adult].

Objective: To explore the differences of PDGF and EGF expression in the wound healing between fatal and adult.

Methods: With the established animal model of fetal scarless healing and the adult samples, an immunohistochemical technique was used to evaluate the expression of PDGF and EGF in the normal adult skin, normal fetal skin, and the process of their wound healing.

Results: 1.

[Expression of basic fibroblast growth factor during wound healing of human fetal and adult skin and its significance].

Objective: To explore the expression of basic fibroblast growth factor(bFGF) during the wound healing of human fetal and adult skin and its significance.

Methods: We established the animal model of fetal scarless healing by transplanting full-thickness skin grafts from human fetus to a subcutaneous location on the athymic mouse recipient, and then making the linear incisions. The expression of bFGF was observed in the normal adult skin, normal fetal skin and during wound healing by immunohistochemical method.

[Expression of hyaluronic acid and its receptor in the process of wound healing in different skin tissues and its significance].

Objective: To explore the expression of hyaluronic acid (HA) and its receptor, Cluster of differentiation 44 (CD44) in proliferative scar and in the process of wound healing of normal human adult skin and fetal skin, and the effect of HA and its receptor on the process of human fetal skin scarless healing.

Methods: An incision and then a hypodermic cavity were made on each side of the dorsal median line of 32 female adult BALB/c rats. Skin grafts from 8 human fetuses delivered by natural abortion, full-thickness skin grafts from 8 normal adults undergoing plastic operation, skin wound sample from the donor sites in legs of 8 patients undergoing dermatoplasty with intermediate split thickness skin graft, and proliferative scar from 8 patients of plastic surgery, non-adult and adult, were grafted into the hypodermic cavities.

[The significance and the expression of homeobox genes during human burn wound healing].

Objective: To investigate the expression of several homeobox genes during the wound healing in fetal and adult skin and their roles in fetal scarless wound healing.

Methods: The expressions of PRX-2, HOXB13, HOX2.2 and HOX2.