[Study on the structure and constituents of polysaccharide from ganoderma lucidum].

Ganoderma lucidum polysaccharides were extracted by boiling water from the fruiting bodies of red ganoderma lucidum, then protein was removed by Sevage reagent, with ethanol precipitation, run water dialysis, centrifugation and freeing-drying. Monosaccharides were acetylated and quantified by external standard, and the polysaccharides the amount of total carbohydrates was determined by phenol-sulfuric acid method. The monosaccharide constituents, structure and Mw distribution were analyzed by UV-Vis, IR, GC, GC-MS and HPSEC with ELSD.