Effect of inhibiting Beclin-1 expression on autophagy, proliferation and apoptosis in colorectal cancer.

The present study aimed to investigate the molecular mechanisms and effect of Beclin-1 on autophagy, proliferation and apoptosis in the colorectal cancer (CRC) HCT116 and SW620 cells. Beclin-1 was silenced by RNA interference (RNAi) in HTC116 and SW620 cells. Reverse transcription-polymerase chain reaction and western blot were used to measure the expression of Beclin-1.

[Study thought of pharmaceutical preparations quality standards by dynamic quality control technology].

Pharmaceutical preparations, particularly as a "secret recipe" of traditional Chinese medicine in medical institutions, are the product of China's medical and health industry, and they are also an important means of competing of different medical institutions. Although pharmaceutical preparations have advantages and characteristics than institutes for drug and pharmaceutical companies, the quality standards of pharmaceutical preparations in medical institutions has not reached the desired level over the years. As we all know, the quality of pharmaceutical preparations is important to ensure the efficacy, especially under the environment of people pay more sttention on drug safety and effectiveness and contry increase emphasis on the stste of pharmaceutical preparations.

[Study on the quality standards of decoction pieces of salt Eucommia].

Objective: To establish the quality criteria of decoction pieces of salt Eucommia.

Methods: Decoction pieces of salt Eucommia were measured with moisture, total ash and alcohol-extract, and were analyzed by TLC and HPLC for research.

Results: We obtained 13 batches of decoction pieces of salt Eucommia moisture, total ash and alcohol-extract measurements; Meanwhile we set the TLC and HPLC method.