[Effects of the Combination of Emodin and 3'-Azido-3'-Deoxythymidine on Proliferation and Apoptosis in leukemia KG-1a cells Transfected with Egr-1 siRNA].

Objective: This study was aimed to investigate the effects of emodin combined with 3'-azido-3'-deoxythymidine (AZT) on proliferation and apoptosis of leukemia cell line KG-1a cells and its mechanism.

Methods: KG-1a cells were transfected with Egr-1 siRNA by electroporation and divided into blank control (KG-1a), nonspecific control (KG-1a/NC) and Egr-1 siRNA (KG-1a/siRNA) groups. Transfection efficiency was tested through fluorescence microscopy and flow cytometry and the transfection effect was detected by using qPCR.

[Effects of AZT on leukemia cell line KG-1a proliferation and telomerase activity].

This study was purposed to investigate the effect of 3'-azido-2', 3'-dideoxythymidine (AZT)on the proliferation and telomerase activity of human acute myeloid leukemia cell line KG-1a. The effect of proliferation was detected by MTT assay after the KG-1a cell were stimulated for 24, 48 and 72 h with different concentrations of AZT; telomerase activity was detected with TRAP-PCR-ELISA assay; RT-PCR was used to detect telomerase hTERT mRNA expression. The results showed that the proliferation of KG-1a cells was inhibited in a time and concentration dependent manner after exposure to AZT for 24, 48 and 72 h; the KG-1a cells decreased in S phase and increased in G(2)/M phase with the increasing of the concentration of AZT; telomerase activity and hTERT-mRNA expression in the experimental groups decreased after treated with AZT, which was positively correlated with concentration of AZT.

[Effects of Clostridium difficile toxin a on proliferation of K562 cells].

This study was aimed to investigate the effect of clostridium difficile toxin A (Tcd A) on proliferation of K562 cells and its mechanism. The proliferative activity of K562 cells exposed to Tcd A was tested by MTT assay; cell cycle distribution and mitochondrial membrane potential were analyzed by flow cytometry; the protein expression of cytochrome C and DNA fragmentation were observed by immunohistochemistry staining and agarose gel electrophoresis respectively. The results indicated that Tcd A inhibited proliferation of K562 cells in a time-and concentration-dependent manner.

[Effect of Tangzhiping granule on blood lipids and free fatty acids in rats with insulin resistant diabetes].

Objective: To investigate the effects of Tangzhiping Granule (TZPG) on blood lipids and free fatty acids (FFA) in rats with insulin resistant diabetes (IRD).

Methods: A blank control group consisted of randomly selected normal rats was set up. The remaining rats were established to IRD model by high-fat high-sugar diet feeding and streptozotocin injection.