Capillary action-driven surface-enhanced Raman spectroscopy (SERS) for the identification of phthalocyanine blue in modern paintings based on the BPG spot test.

A method for the extraction and accumulation of Ag nanoparticles utilizing capillary action through a piece of triangular-shaped cotton paper is reported. This method allowed the integration of SERS and the BPG spot test, which are typically employed for examining pigments on paper or in books. Malachite green oxalate was used as the model material for investigating the extent of enhancement when SERS was applied.

The use of RGB-tracking of color changes during indigo-reduction processes based on LabVIEW machine vision.

The use of an RGB-tracking chart for monitoring the reduction of indigo (color changes) based on the LabVIEW machine vision is demonstrated for the first time. In contrast to a normal analytical chromatographic chart, the time scale is used on the X-axis, but the sum of "RGB-pixels" is used on the Y-axis, instead of "signal intensity". The RGB-tracking chart was obtained from an investigation of the process involved in the reduction of indigo, in which a PC camera was used as a detector and LabVIEW machine vision was simultaneously operated.

Selection of aptamers targeting the sialic acid receptor of hemagglutinin by epitope-specific SELEX.

A new SELEX scheme is proposed for the selection of aptamers targeting a specific epitope of a native protein. Anti-sialic acid receptor (SAR) aptamers that inhibit H1 hemagglutination at a low picomole dose are selected accordingly.

Analysis of ephedra-alkaloids using sweeping and cation-selective exhaustive injection and sweeping micellar electrokinetic chromatography methods.

Two stacking methods of capillary electrophoresis (CE) were developed for the separation of very dilute solutions of ephedra-alkaloids, namely ephedrine, pseudoephedrine, methylephedrine, methylpseudoephedrine, norephedrine, and norpseudoephedrine. A sweeping method which uses a carrier comprised of phosphoric acid, sodium dodecyl sulfate (SDS), diethylamine and acetonitrile permits the detection of the alkaloids down to the 10(-1) microg/mL level, and the cation-selective exhaustive injection and sweeping micellar electrokinetic chromatography (CSEI-Sweep-MEKC) method using phosphoric acid, SDS, and acetronitrile as electrolytes can detect down to the 10(-3) microg/mL level. The former requires the conductance of the sample solution to be adjusted beforehand, and only five peaks were observed, two of which were overlapped.

Sub-diffraction-limit imaging based on the topographic contrast of differential confocal microscopy.

Using the nanometer depth sensitivity of differential confocal microscopy, we detect surface features of lateral dimensions smaller than the diffraction limit without fluorescence labeling. The lateral resolution of the topographic images is further enhanced by a maximum-likelihood estimation algorithm. Based on the comparison of signal and noise at high spatial frequency, we estimate the best lateral resolution of the enhanced images to be 0.